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首页> 外文期刊>Optical and quantum electronics >Semi-quantitative Surface Enhanced Raman Scattering Spectroscopic Creatinine Measurement in Human Urine Samples
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Semi-quantitative Surface Enhanced Raman Scattering Spectroscopic Creatinine Measurement in Human Urine Samples

机译：人尿液样品中的半定量表面增强拉曼散射光谱肌酐测定

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摘要

This paper presents the development of a semi-quantitative method of measuring the creatinine biomolecule in human urine by the surface enhanced Raman scattering (SERS) technique. Creatinine is one of the major components of urine and can be used to represent the metabolic and renal function of the human body. The Raman signal of creatinine is enhanced by 50 nm Au nanoparticles. Raman spectra between 1400 and 1500 cm~(-1) were analyzed to obtain the relationship between the SERS band area and creatinine concentration. The square of the correlation coefficient is 0.99 in artificial urine over the creatinine range 38.4-153.6 mg/dl. In a human urine experiment, a good linear correlation is observed over the creatinine concentration range 2.56-6.4 mg/dl. The square of correlation coefficient is 0.96.
机译：本文提出了一种通过表面增强拉曼散射（SERS）技术测量人尿中肌酐生物分子的半定量方法的开发。肌酐是尿液的主要成分之一，可用于代表人体的代谢和肾脏功能。肌酐的拉曼信号被50 nm Au纳米颗粒增强。分析了1400〜1500 cm〜（-1）之间的拉曼光谱，得出SERS谱带面积与肌酐浓度之间的关系。在肌酐范围为38.4-153.6 mg / dl的人造尿液中，相关系数的平方为0.99。在人类尿液实验中，在肌酸酐浓度范围2.56-6.4 mg / dl处观察到良好的线性相关性。相关系数的平方为0.96。

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