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Coherent anti-Stokes Raman scattering microscopy using tightly focused radially polarized light

机译:使用紧密聚焦的径向偏振光的相干抗斯托克斯拉曼散射显微镜

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摘要

We report a radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy for facilitatingnlongitudinally oriented molecules detection. We observe that under tight focusing of radially polarized pumpnand Stokes light fields with a high-NA objective, RP-CARS radiation from molecules oriented along the lon-ngitudinal direction is approximately threefold stronger than that using linearly polarized CARS (LP-CARS)ntechnique. The lateral resolution of RP-CARS imaging can be improved by about 10% compared to the LP-nCARS imaging. We demonstrate this RP-CARS technique by imaging the sectioned cottonwood leaf vascularnbundles. © 2009 Optical Society of America
机译:我们报告径向极化相干抗斯托克斯拉曼散射(RP-CARS)显微镜,以促进nlongitudinally定向分子检测。我们观察到,在具有高NA物镜的径向偏振泵浦光和斯托克斯光场的严格聚焦下,沿纵向方向定向的分子的RP-CARS辐射强度比使用线性偏振CARS(LP-CARS)技术的强度强大约三倍。与LP-nCARS成像相比,RP-CARS成像的横向分辨率可提高约10%。我们通过对切片的白杨叶维管束进行成像来证明该RP-CARS技术。 ©2009美国眼镜学会

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  • 来源
    《Optics Letters》 |2009年第12期|p.1870-1872|共3页
  • 作者单位

    Optical Bioimaging Laboratory, Department of Bioengineering, Faculty of Engineering,National University of Singapore, Singapore 117576* Corresponding author: biehzw@nus.edu.sg;

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