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首页> 外文期刊>Optics Letters >Axial coding in full-field microscopy using three-dimensional structured illumination implemented with no moving parts
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Axial coding in full-field microscopy using three-dimensional structured illumination implemented with no moving parts

机译:使用没有移动部件的三维结构照明在全尺寸显微镜中进行轴编码

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摘要

We report a simple optical setup to produce both axial and lateral structured illumination through a singlenobjective lens. With a minimum of six full-field images obtained without moving either the sample or thenmicroscope objective, 100 nm diameter fluorescent beads can be localized axially with an accuracy of 50 nmnin a 1.76-u0001m-thick layer. We show that this axial localization improvement can easily be combined withnclassical lateral structured illumination, so that lateral resolution enhancement by a factor of 2 isnmaintained. © 2008 Optical Society of America
机译:我们报告了一个简单的光学装置,可通过单物镜产生轴向和横向结构照明。在不移动样品或显微镜物镜的情况下获得最少六张全图图像,可以在1.76-u0001m厚的层中以100 nm的直径轴向定位100 nm直径的荧光珠。我们表明,这种轴向定位的改进可以很容易地与经典的横向结构照明相结合,从而使横向分辨率提高了2倍。 ©2008美国眼镜学会

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