Hyperspectral in vivo two-photon microscopy of intrinsic contrast

Andrew J. Radosevich Matthew B. Bouchard Sean A. Burgess Brenda R. Chen and Elizabeth M. C. Hillman*

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Hyperspectral in vivo two-photon microscopy of intrinsic contrast

机译：高光谱体内双光子显微镜的固有对比度

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In vivo two-photon imaging of intrinsic contrast can provide valuable information about structural tissuenelements such as collagen and elastin and ﬂuorescent metabolites such as nicotinamide adenine dinucle-notide. Yet low signal and overlapping emission spectra can make it difﬁcult to identify and delineate thesenspecies in vivo. We present a novel approach that combines excitation scanning with spectrally resolvednemission two-photon microscopy, allowing distinct structures to be delineated based on their characteristicnspectral ﬁngerprints. The amounts of intrinsic ﬂuorophores present in each voxel can also be evaluated. Wendemonstrate our method using in vivo imaging of nude mouse skin. © 2008 Optical Society of America

机译：体内固有对比度的双光子成像可以提供有关结构组织元素（例如胶原蛋白和弹性蛋白）和荧光代谢物（例如烟酰胺腺嘌呤二核苷）的有价值的信息。然而，低信号和重叠的发射光谱可能使其难以在体内鉴定和勾画出物种。我们提出了一种新颖的方法，将激发扫描与光谱分辨发射双光子显微镜相结合，允许根据其特征光谱指纹来描绘不同的结构。也可以评估每个体素中存在的固有荧光团的数量。使用裸鼠皮肤的体内成像演示我们的方法。 ©2008美国眼镜学会

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《Optics Letters》 |2008年第18期|p.2164-2166|共3页
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Andrew J. Radosevich Matthew B. Bouchard Sean A. Burgess Brenda R. Chen and Elizabeth M. C. Hillman*;
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Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, ColumbiaUniversity, 1210 Amsterdam Avenue, New York, New York 10027, USA* Corresponding author: eh2245@columbia.edu;

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1. Hyperspectral in vivo two-photon microscopy of intrinsic contrast [J] . Andrew J. Radosevich, Matthew B. Bouchard, Sean A. Burgess, Optics Letters . 2008,第18期

机译：高光谱体内双光子显微镜的固有对比度
2. Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution [J] . Szu-Yu Chen, Chiao-Sheng Lu, Chia-Hua Yeh Biomedical Optics Express . 2014,第2期

机译：具有高光谱和空间分辨率的非扫描平行记录双光子高光谱显微镜
3. Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution [J] . Szu-Yu Chen, Chiao-Sheng Lu, Chia-Hua Yeh Biomedical Optics Express . 2014,第2期

机译：具有高光谱和空间分辨率的非扫描平行记录双光子高光谱显微镜
4. In vivo track the development of melanoma with the intrinsic third harmonic generation and two-photon fluorescence contrasts of melanin [C] . Pei-Chun Wu, Yu-Shing Chen, Tsung-Yuan Hsieh, Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications IV . 2012

机译：体内通过固有的三次谐波产生和黑色素的双光子荧光对比来追踪黑色素瘤的发展
5. Development of contrast enhanced MR microscopy for visualization of brain tumor invasion in vivo. [D] . Bahk, Kenneth Dae. 2002

机译：对比增强MR显微镜的发展，用于可视化体内脑肿瘤的侵袭。
6. Hyperspectral in vivo two-photon microscopy of intrinsic contrast [O] . Andrew J. Radosevich, Matthew B. Bouchard, Sean A. Burgess, -1

机译：高光谱体内双光子显微镜的固有对比度
7. Spectral Characterization and Unmixing of Intrinsic Contrast in Intact Normal and Diseased Gastric Tissues Using Hyperspectral Two-Photon Microscopy [O] . Grosberg, Lauren E., Radosevich, Andrew J., Asfaha, Samuel, 2011

机译：使用高光谱双光子显微镜对完整的正常和患病胃组织中的固有光谱进行光谱表征和混合

Hyperspectral in vivo two-photon microscopy of intrinsic contrast

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