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In vivo track the development of melanoma with the intrinsic third harmonic generation and two-photon fluorescence contrasts of melanin

机译:体内通过固有的三次谐波产生和黑色素的双光子荧光对比来追踪黑色素瘤的发展

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The understanding of the interaction between tumors and surrounding microenvironment in vivo is an important first step and basis for pathway-targeting cancer therapy. To in vivo observe the dynamic development of tumor cells and validate the efficacy of therapy in microscopic scales, people commonly performed multi-photon fluorescence microscopy through an invasive window chamber setup. However, under such system, the cancer cells can't be identified and long-term tracked without a fluorescence labeling. Exploiting the intrinsic third harmonic generation (THG) and two-photon fluorescence (2PF) contrasts of melanin, we demonstrated in vivo identification of melanoma and tracked its development without labeling. It was achieved with a least invasive femtosecond Cnforsterite laser and a laser scanning nonlinear microscopy system with 3D sub-micron spatial resolution. Combined with molecular probes or reporters, we anticipate thus developed platform a powerful tool to reveal molecular insights of tumor microenvironments, enhance our understanding of tumor biology, and trigger new therapeutic approaches.
机译:体内肿瘤与周围微环境之间相互作用的理解是靶向途径癌症治疗的重要第一步和基础。为了在体内观察肿瘤细胞的动态发展并在微观尺度上验证治疗的有效性,人们通常通过侵入性窗口室设置进行多光子荧光显微镜检查。但是,在这样的系统下,没有荧光标记就无法识别和长期追踪癌细胞。利用黑色素的固有的三次谐波产生(THG)和双光子荧光(2PF)对比,我们证明了体内对黑色素瘤的鉴定,并在未标记的情况下跟踪了它的发展。它是通过微创飞秒Cnforsterite激光和具有3D亚微米空间分辨率的激光扫描非线性显微镜系统实现的。结合分子探针或报道分子,我们预计由此开发的平台将成为一个强大的工具,可揭示对肿瘤微环境的分子见解,增进对肿瘤生物学的了解并触发新的治疗方法。

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