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首页> 外文期刊>Scanning >Multiphoton Microscopic Imaging of In Vivo Hair Mouse Skin Based on Two-Photon Excited Fluorescence and Second Harmonic Generation
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Multiphoton Microscopic Imaging of In Vivo Hair Mouse Skin Based on Two-Photon Excited Fluorescence and Second Harmonic Generation

机译：基于双光子激发荧光和二次谐波产生的体内毛发小鼠皮肤的多光子显微成像

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摘要

Mouse is an important animal model to investigate skin physiological and pathological states. In this article, multiphoton microscopic imaging of in vivo hair mouse skin based on two-photon excited fluorescence and second harmonic generation was examined. Our results show that multiphoton microscopy can clearly display micro-structure of stratum corneum, stratum spinosum, and dermis of in vivo mouse skin. The main components of epidermis and dermis such as corneo-cytes, spinosum cell, collagen fibers, and hair follicles can be distinctly identified in MPM images. Using the optional HRZ 200fine focusing stage, thickness of different layers can be easily assessed. The results demonstrate that MPM can be regarded as an efficient method for in vivo investigation of skin physiological and pathological states by using hair mouse animal model
机译：小鼠是研究皮肤生理和病理状态的重要动物模型。在本文中，研究了基于双光子激发荧光和二次谐波产生的体内毛小鼠皮肤的多光子显微成像。我们的结果表明，多光子显微镜可以清楚显示体内小鼠皮肤的角质层，棘层和真皮层的微观结构。可以在MPM图像中清楚地识别表皮和真皮的主要成分，例如角质细胞，棘皮细胞，胶原纤维和毛囊。使用可选的HRZ 200fine聚焦镜台，可以轻松评估不同层的厚度。结果表明，通过使用毛发小鼠动物模型，MPM可被视为体内研究皮肤生理和病理状态的有效方法。

著录项

  • 来源
    《Scanning》 |2012年第3期|p.170-173|共4页
  • 作者

    Xingshan Jiang; Shuangmu Zhuo; Renan Xu; Jianxin Chen;

  • 作者单位

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    multiphoton microscopy (MPM); second harmonic generation (SHG); two-photon excited fluorescence (2PEF); in vivo hair mouse skin;

    机译：多光子显微镜（MPM）;二次谐波（SHG）双光子激发荧光（2PEF）;体内毛小鼠皮肤;

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