Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence

Aikaterini Zoumi; Alvin Yeh; Bruce J. Tromberg

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Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence

机译：通过二次谐波产生和双光子激发荧光对体内细胞和细胞外基质成像

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Multiphoton microscopy relies on nonlinear light-matter interactions to provide contrast and optical sectioning capability for high-resolu- tion imaging. Most multiphoton microscopy studies in biological systems have relied on two-photon excited fluorescence (TPEF) to produce images. With increasing applications of multiphoton micros- copy to thick-tissue "intravital" imaging, second-harmonic genera- tion (SHG) from structural proteins has emerged as a potentially important new contrast mechanism.

机译：多光子显微镜依靠非线性光-物质相互作用为高分辨率成像提供对比度和光学切片能力。生物系统中的大多数多光子显微镜研究都依靠双光子激发荧光（TPEF）产生图像。随着多光子显微技术在厚组织“活体”成像中的应用不断增加，由结构蛋白产生的第二谐波产生（SHG）已成为一种潜在的重要的新对比机制。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2002年第17期|p.11014-11019|共6页
作者
Aikaterini Zoumi; Alvin Yeh; Bruce J. Tromberg;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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5. Synthesis, characterization, and evaluation of new reactive two-photon absorbing dyes for two-photon excited fluorescence imaging applications [D] . Schafer, Katherine J. 2005

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6. Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence [O] . Aikaterini Zoumi, Alvin Yeh, Bruce J. Tromberg 2002

机译：通过二次谐波产生和双光子激发荧光对体内细胞和细胞外基质成像
7. Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence [O] . Zoumi, Aikaterini, Yeh, Alvin, Tromberg, Bruce J. 2002

机译：通过二次谐波产生和双光子激发荧光对体内细胞和细胞外基质成像

Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence

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