The viral RNA-based transfection of enhanced green fluorescent protein (EGFP) in the parasitic protozoan Trichomonas vaginalis

Wei Li; He Ding; Xinxin Zhang; Lili Cao; Jianhua Li; Pengtao Gong; He Li; Guocai Zhang; Shuhong Li; Xichen Zhang

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The viral RNA-based transfection of enhanced green fluorescent protein (EGFP) in the parasitic protozoan Trichomonas vaginalis

机译：基于病毒RNA的转染增强型绿色荧光蛋白（EGFP）在寄生原生动物阴道毛滴虫

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Here we have developed methods to transiently and stably transfect the human pathogenic protist Trichomonas vaginalis. The viral RNA-based transfection vector pTVV-EGFP/NEO was constructed by using enhanced green fluorescent protein gene (EGFP) and neomycin resistance gene (NEO) in tandem to replace the whole gene encoding region of T. vaginalis virus (TVV). The in vitro transcripts of linearized pTVV-EGFP/NEO were electroporated into trophozoites and the transfectants transiently expressed EGFP after 16 h postincubation. Stable expression of EGFP was persistently detected by fluorescence microscopy and by RT-PCR in transfected trophozoites under G418 selection. Our study provides a novel and valuable approach for genetic study of T. vaginalis.

机译：在这里，我们已经开发出可以瞬时稳定地转染人类病原菌阴道毛滴虫的方法。串联使用增强的绿色荧光蛋白基因（EGFP）和新霉素抗性基因（NEO）取代阴道锥状病毒（TVV）的整个基因编码区，构建了基于病毒RNA的转染载体pTVV-EGFP / NEO。将线性化的pTVV-EGFP / NEO的体外转录本电穿孔入滋养体，孵育16小时后，转染子瞬时表达EGFP。在G418选择下，通过荧光显微镜和RT-PCR在转染的滋养体中持续检测到EGFP的稳定表达。我们的研究为阴道锥虫的遗传研究提供了一种新颖而有价值的方法。

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《Parasitology Research》 |2012年第3期|p.1305-1310|共6页
作者
Wei Li; He Ding; Xinxin Zhang; Lili Cao; Jianhua Li; Pengtao Gong; He Li; Guocai Zhang; Shuhong Li; Xichen Zhang;
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1. The viral RNA-based transfection of enhanced green fluorescent protein (EGFP) in the parasitic protozoan Trichomonas vaginalis. [J] . Wei Li, He Ding, Xinxin Zhang, Parasitology Research . 2012,第3期

机译：基于病毒RNA的转染增强型绿色荧光蛋白（EGFP）在寄生原生动物阴道毛滴虫中。
2. Viral RNA-based transfection and expression of enhanced green fluorescent protein in the parasitic protozoan Eimeria stiedae [J] . Caiyan Xin, Jianhua Li, Pengtao Gong, 生物化学与生物物理学报：英文版 . 2019,第002期

机译：基于病毒RNA的转染和增强的绿色荧光蛋白在寄生原生动物艾美球虫中的表达
3. The fluorescent protein iLOV outperforms eGFP as a reporter gene in the microaerophilic protozoan Trichomonas vaginalis [J] . Shuqi E. Wang, Anna E.S. Brooks, Bronwyn Cann, Molecular and Biochemical Parasitology . 2017,第期

机译：荧光蛋白ILOV以微生罗基原生动物Trichomonas阴道中的报道基因优于EGFP
4. Single molecule surface enhanced resonance Raman scattering (SERRS) of the enhanced green fluorescent protein (EGFP) [C] . J. Hofkens, F.C. De Schryver, M. Cotlet, . 2004

机译：增强的绿色荧光蛋白（EGFP）的单分子表面增强的共振拉曼散射（SERRS）
5. Using proteomics and CRISPR to identify key host regulatory proteins involved in host-parasite interactions following the attachment of Trichomonas vaginalis [D] . Tsiotsioras, George 2017

机译：使用蛋白质组学和CRISPR鉴定阴道毛滴虫附着后参与宿主-寄生虫相互作用的关键宿主调节蛋白
6. The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo [O] . Jo E. Lewis, John M. Brameld, Phil Hill, -1

机译：病毒2A序列在体外和体内同时过表达vgf基因和增强型绿色荧光蛋白（eGFP）的用途
7. The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP [O] . Lewis Jo E., Brameld John M., Hill P.J., 2015

机译：使用病毒2A序列同时表达vgf基因和增强型绿色荧光蛋白eGFP

The viral RNA-based transfection of enhanced green fluorescent protein (EGFP) in the parasitic protozoan Trichomonas vaginalis

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