Real-time quantitative reverse transcription-PCR analysis of expression stability of Aggregatibacter actinomycetemcomitans fimbria-associated gene in response to photodynamic therapy

Pourhajibagher Maryam; Monzavi Abbas; Chiniforush Nasim; Monzavi Mohammad Moein; Sobhani Shaghayegh; Shahabi Sima; Bahador Abbas

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Real-time quantitative reverse transcription-PCR analysis of expression stability of Aggregatibacter actinomycetemcomitans fimbria-associated gene in response to photodynamic therapy

机译：实时定量逆转录-PCR分析光动力疗法对放线杆菌聚合纤维相关基因表达稳定性的影响

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Background: Aggregatibacter actinomycetemcomitans is an etiological agent of both chronic and aggressive periodontitis. Dissemination of A. actinomycetemcomitans from the oral cavity and initiation of systemic infections has led to new approaches for treatment being needed. In this study, a series of experiments presented investigated the effect of methylene blue (MB)-mediated antimicrobial photodynamic therapy (aPDT) on cell viability and expression of fimbria-associated gene (rcpA) in A. actinomycetemcomitans.

机译：背景：放线杆菌聚合酶是慢性和侵袭性牙周炎的病因。从口腔中传播放线放线杆菌并引发全身感染已经导致需要新的治疗方法。在这项研究中，提出了一系列实验，研究了亚甲基蓝（MB）介导的抗微生物光动力疗法（aPDT）对拟南芥放线菌中细胞活力和菌毛相关基因（rcpA）表达的影响。

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来源
《Photodiagnosis and Photodynamic Therapy》 |2017年第6期|78-82|共5页
作者
Pourhajibagher Maryam; Monzavi Abbas; Chiniforush Nasim; Monzavi Mohammad Moein; Sobhani Shaghayegh; Shahabi Sima; Bahador Abbas;
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作者单位

Univ Tehran Med Sci, Sch Med, Dept Microbiol, Tehran, Iran;

Univ Tehran Med Sci, Dent Res Inst, Laser Res Ctr, Tehran, Iran;

Univ Tehran Med Sci, Dent Res Inst, Laser Res Ctr, Tehran, Iran;

Univ Tehran Med Sci, Sch Dent, Tehran, Iran;

Univ Tehran Med Sci, Sch Dent, Tehran, Iran;

Univ Tehran Med Sci, Dent Res Inst, Laser Res Ctr, Tehran, Iran;

Univ Tehran Med Sci, Sch Med, Dept Microbiol, Tehran, Iran|Univ Tehran Med Sci, Dent Res Inst, Laser Res Ctr, Tehran, Iran|Univ Tehran Med Sci, Dent Res Inst, Dent Res Ctr, Tehran, Iran;

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正文语种 eng
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关键词
Aggregatibacter actinomycetemcomitans; Biofilm; Diode laser; Methylene blue; Photodynamic therapy; qRT-PCR; rcpA; Virulence factor;

机译：聚合酶放线菌;生物膜;二极管激光;亚甲基蓝;光动力疗法;qRT-PCR;rcpA;毒力因子;

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1. Monitoring gene expression of rcpA from Aggregatibacter actinomycetemcomitans versus antimicrobial photodynamic therapy by relative quantitative real-time PCR [J] . Pourhajibagher Maryam, Chiniforush Nasim, Shahabi Sima, Photodiagnosis and Photodynamic Therapy . 2017,第sepa期

机译：相对定量实时PCR监测放线杆菌聚合酶与抗菌光动力学疗法对rcpA的基因表达
2. Real-Time Quantitative Reverse Transcription-PCR Analysis of Expression Stability of Actinobacillus pleuropneumoniae Housekeeping Genes during In Vitro Growth under Iron-Depleted Conditions [J] . K. Klitgaard Nielsen, M. Boye Applied and Environmental Microbiology . 2005,第6期

机译：贫铁条件下胸膜肺炎放线杆菌管家基因体外生长过程中表达稳定性的实时定量逆转录-PCR分析
3. Evaluation of internal reference genes for quantitative expression analysis by real-time reverse transcription-PCR in somatic cells from goat milk [J] . P. Modesto, S. Peletto, G. Pisoni, Journal of dairy science . 2013,第12期

机译：通过实时逆转录-PCR评估山羊奶体细胞中内部参考基因的定量表达分析
4. Interleukin-12 reverses the inhibitory impact of photodynamic therapy (PDT) on the murine contact hypersensitivity response [C] . Guillermo O. Simkin, QLT PhotoTherapeutics, Inc., Optical Methods for Tumor Treatment and Detections: Mechanisms and Techniques in Photodynamic Therapy VII . 1998

机译：白介素12逆转光动力疗法（PDT）对小鼠接触超敏反应的抑制作用
5. Quantitative Analysis of Aggregatibacter actinomycetemcomitans in Dental Plaque Samples of Moroccan School Children with and Without Periodontitis [D] . Kum, John M. 2019

机译：无牙周炎摩洛哥学科儿童牙科斑块样品中聚合物斑块样品的定量分析
6. Real-Time Quantitative Reverse Transcription-PCR Analysis of Expression Stability of Actinobacillus pleuropneumoniae Housekeeping Genes during In Vitro Growth under Iron-Depleted Conditions [O] . K. Klitgaard Nielsen, M. Boye 2005

机译：贫铁条件下胸膜肺炎放线杆菌管家基因体外生长过程中表达稳定性的实时定量逆转录-PCR分析
7. Real-Time Quantitative Reverse Transcription-PCR Analysis of Expression Stability of Actinobacillus pleuropneumoniae Housekeeping Genes during In Vitro Growth under Iron-Depleted Conditions [O] . Nielsen, K. Klitgaard, Boye, M. 2005

机译：贫铁条件下胸膜肺炎放线杆菌管家基因体外生长过程中表达稳定性的实时定量逆转录-PCR分析

Real-time quantitative reverse transcription-PCR analysis of expression stability of Aggregatibacter actinomycetemcomitans fimbria-associated gene in response to photodynamic therapy

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