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Eosin fluorescence: A diagnostic tool for quantification of liver injury

机译：曙红荧光：定量肝损伤的诊断工具

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摘要

Hepatitis is one of the most common life threatening diseases. The diagnosis is mainly based on biochemical analysis such as liver function test. However, histopathological evaluation of liver serves far better for more accurate final diagnosis. The goal of our study was to evaluate the eosin fluorescence pattern in CCl4-induced liver injury model compared with normal and different treatment groups. For this purpose, liver tissues were stained with H/E and examined under bright field microscope but the fluorescence microscopy of FIX stained slides provided an interesting fluorescence pattern and was quite helpful in identifying different structures. Interesting fluorescence patterns were obtained with FITC, Texas Red and Dual channel filter cubes that were quite helpful in identifying different morphological features of the liver. During the course of hepatic injury, liver cells undergo necrosis, apoptosis and overall cellular microenvironment is altered due to the modification of proteins and other intracellular molecules. Intensified eosin fluorescence was observed around the central vein of injured liver compared to normal indicating enhanced binding of eosin to the more exposed amino acid residues. To conclude, eosin fluorescence pattern varies with the health status of a tissue and can be used further for the diagnosis and quantification of severity of various liver diseases. (C) 2017 Elsevier B.V. All rights reserved.
机译：肝炎是最常见的威胁生命的疾病之一。诊断主要基于肝功能检查等生化分析。但是，肝脏的组织病理学评估可以更好地进行更准确的最终诊断。我们研究的目的是评估与正常和不同治疗组相比，CCl4诱导的肝损伤模型中曙红的荧光模式。为此，用H / E对肝脏组织进行染色，并在明场显微镜下进行检查，但是FIX染色载玻片的荧光显微镜提供了有趣的荧光图案，对鉴定不同的结构非常有帮助。使用FITC，Texas Red和Dual通道滤光片立方体获得了有趣的荧光图案，这对于识别肝脏的不同形态特征非常有帮助。在肝损伤过程中，肝细胞会发生坏死，凋亡，并且由于蛋白质和其他细胞内分子的修饰而改变了整个细胞的微环境。与正常人相比，在受伤的肝脏中心静脉周围观察到曙红荧光增强，表明曙红与更多暴露的氨基酸残基的结合增强。总之，曙红荧光图谱随组织的健康状况而变化，并可进一步用于各种肝病严重程度的诊断和定量。 （C）2017 Elsevier B.V.保留所有权利。

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