• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Photodynamic action of palmatine hydrochloride on colon adenocarcinoma HT-29 cells
【24h】

Photodynamic action of palmatine hydrochloride on colon adenocarcinoma HT-29 cells

机译：盐酸棕榈碱对结肠腺癌HT-29细胞的光动力作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Palmatine hydrochloride (PaH) is a natural active compound from a traditional Chinese medicine (TCM). The present study aims to evaluate the effect of PaH as a new photosensitizer on colon adenocarcinoma HT-29 cells upon light irradiation. Firstly, the absorption and fluorescence spectra of PaH were measured using a UV-vis spectrophotometer and RF-1500PC spectrophotometer, respectively. Singlet oxygen (O-1(2)) production of PaH was determined using 1, 3-diphenylisobenzofuran (DPBF). Dark toxicity of PaH was estimated using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MIT) assay. Cellular uptake of PaH in HT-29 cells was detected at different time intervals. Subellular localization of PaH in HT-29 cells was observed using confocal laser fluorescence microscopy. For photodynamic treatment, HT-29 cells were incubated with PaH and then irradiated by visible light (470 nm) from a LED light source. Photocytotoxicity was investigated 24 h after photodynamic treatment using MIT assay. Cell apoptosis was observed 18 h after photodynamic treatment using a flow cytometry with Annexin V/PI staining. Results showed that PaH has an absorption peak in the visible region from 400 nm to 500 nm and a fluorescence emission peak at 406 nm with an excitation wavelength of 365 nm. PaH was activated by the 470 nm visible light from a LED light source to produce O-1(2). Dark toxicity showed that PaH alone treatment had no cytotoxicity to HT-29 cancer cells and NIH-3T3 normal cells after incubation for 24 h. After incubation for 40 min, the cellular uptake of PaH reached to the maximum and PaH was located in mitochondria. Photodynamic treatment of PaH demonstrated a significant photocytotoxicity on HT-29 cells. The rate of cell death increased significantly in a PaH concentration-dependent and light dose-dependent manner. Further evaluation revealed that the early and late apoptotic rate of HT-29 cells increased remarkably up to 21.54% and 5.39% after photodynamic treatment of PaH at the concentration of 5 mu M and energy density of 10.8 J/cm(2). Our findings demonstrated that Pali as a naturally occurring photosensitizer has potential in photodynamic therapy on colon adenocarcinoma. (C) 2016 Elsevier B.V. All rights reserved.
机译：盐酸帕马汀（PaH）是来自传统中药（TCM）的天然活性化合物。本研究旨在评估PaH作为新型光敏剂对光照射后结肠腺癌HT-29细胞的作用。首先，分别使用紫外可见分光光度计和RF-1500PC分光光度计测量PaH的吸收光谱和荧光光谱。使用1，3-二苯基异苯并呋喃（DPBF）测定PaH的单线态氧（O-1（2））产生。使用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物（MIT）分析评估PaH的暗毒性。在不同的时间间隔检测到HT-29细胞中PaH的细胞摄取。使用共聚焦激光荧光显微镜观察到HT-29细胞中PaH的亚细胞定位。为了进行光动力处理，将HT-29细胞与PaH孵育，然后用来自LED光源的可见光（470 nm）照射。在光动力处理后24小时使用MIT测定法研究光细胞毒性。光动力学处理后18小时，使用膜联蛋白V / PI染色的流式细胞仪观察细胞凋亡。结果表明，PaH在400nm至500nm的可见光区域具有吸收峰，并且在365nm的激发波长下在406nm具有荧光发射峰。 PaH被来自LED光源的470 nm可见光激活，从而产生O-1（2）。黑暗毒性表明，单独孵育24小时后，单独用PaH处理对HT-29癌细胞和NIH-3T3正常细胞没有细胞毒性。孵育40分钟后，细胞对PaH的吸收达到最大，并且PaH位于线粒体中。 PaH的光动力处理显示对HT-29细胞具有明显的光细胞毒性。细胞死亡率以PaH浓度依赖性和光剂量依赖性方式显着增加。进一步的评估表明，在5μM的浓度和10.8J / cm的能量密度下光动力学处理PaH后，HT-29细胞的早期和晚期凋亡率显着增加，分别达到21.54％和5.39％。我们的研究结果表明，作为天然光敏剂的Pali在结肠腺癌的光动力疗法中具有潜力。 （C）2016 Elsevier B.V.保留所有权利。

著录项

  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2016年第9期|53-58|共6页
  • 作者

    Wu Juan; Xiao Qicai; Zhang Na; Xue Changhu; Leung Albert Wingnang; Zhang Hongwei; Xu Chuanshan; Tang Qing-Juan;

  • 作者单位

    Chinese Univ Hong Kong, Fac Med, Sch Chinese Med, Shatin, Hong Kong, Peoples R China|Ocean Univ China, Coll Food Sci & Engn, Lab Food Sci & Human Hlth, Qingdao, Peoples R China;

    Chinese Univ Hong Kong, Fac Med, Sch Chinese Med, Shatin, Hong Kong, Peoples R China;

    Ocean Univ China, Coll Food Sci & Engn, Lab Food Sci & Human Hlth, Qingdao, Peoples R China;

    Ocean Univ China, Coll Food Sci & Engn, Lab Food Sci & Human Hlth, Qingdao, Peoples R China;

    Chinese Univ Hong Kong, Fac Med, Sch Chinese Med, Shatin, Hong Kong, Peoples R China;

    Chinese Univ Hong Kong, Fac Med, Sch Chinese Med, Shatin, Hong Kong, Peoples R China;

    Chinese Univ Hong Kong, Fac Med, Sch Chinese Med, Shatin, Hong Kong, Peoples R China;

    Ocean Univ China, Coll Food Sci & Engn, Lab Food Sci & Human Hlth, Qingdao, Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Palmatine hydrochloride; Photodynamic therapy; Colon adenocarcinoma; Singlet oxygen; Cell apoptosis;

    机译：盐酸帕马汀;光动力疗法;结肠腺癌;单氧;细胞凋亡;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号