• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Enhancing the effect of 5-aminolevulinic acid based photodynamic therapy in human meningioma cells
【24h】

Enhancing the effect of 5-aminolevulinic acid based photodynamic therapy in human meningioma cells

机译：增强基于5-氨基乙酰丙酸的光动力疗法在人脑膜瘤细胞中的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: The interest in photodynamic therapy (PDT) in cancer treatment has continuously increased since its' advent in the 1960s. In neurosurgery, 5-aminolevulinic acid based photodynamic therapy (5-ALA PDT) is used for malignant glioma in vitro and in vivo. Data about PDT in meningioma, which is the second most frequent primary brain tumor, is sparse. Preliminary work indicated that meningiomas are more resistant to PDT than gliomas. In the present study we evaluated whether the effect of 5-ALA PDT on malignant meningioma cells can be increased by ciprofloxacin and changes in incubation time, factors known to influence the effectiveness of PDT in other tumor entities. Methods: An in vitro-PDT model of a malignant human meningioma cell line (KT21-MG) was chosen as screening method. Cultured cells were incubated with varying 5-ALA concentrations for 4h and exposed to PDT (control group). Two experimental groups, one with 5-ALA and ciprofloxacin (group Ⅰ) and one with 24-h ALA incubation time (group Ⅱ), were studied, respectively. Cell viability was assessed by WST-1 assay. Results: Ciprofloxacin and longer ALA incubation time significantly increased the lethal effect of 5-ALA PDT on meningioma cells. Conclusions: Efficacy of 5-ALA PDT could be increased by adjunction of ciprofloxacin in conventional clinical dosing and by prolongation of ALA incubation time. As ciprofloxacin is a common antibiotic with good tissue penetration, low toxicity and a favorable risk profile it represents a clinically interesting method for future PDT trials in meningiomas.
机译：背景：自1960年代问世以来，对光动力疗法（PDT）在癌症治疗中的兴趣一直在不断增长。在神经外科中，基于5-氨基乙酰丙酸的光动力疗法（5-ALA PDT）在体外和体内用于恶性神经胶质瘤。脑膜瘤中PDT的数据稀疏，后者是脑膜瘤中第二常见的肿瘤。初步工作表明，脑膜瘤比胶质瘤对PDT更具抵抗力。在本研究中，我们评估了环丙沙星和潜伏期的改变是否可以提高5-ALA PDT对恶性脑膜瘤细胞的作用，而潜伏期的改变是影响PDT在其他肿瘤实体中有效性的因素。方法：选择人脑膜瘤细胞系（KT21-MG）的体外PDT模型作为筛选方法。将培养的细胞与不同浓度的5-ALA孵育4小时，然后暴露于PDT（对照组）。研究了两个实验组，一个是5-ALA和环丙沙星（Ⅰ组），另一个是24小时ALA温育时间（Ⅱ组）。通过WST-1测定法评估细胞活力。结果：环丙沙星和更长的ALA孵育时间显着增加了5-ALA PDT对脑膜瘤细胞的致死作用。结论：环丙沙星在常规临床给药中的辅助作用和延长ALA孵育时间可提高5-ALA PDT的疗效。由于环丙沙星是一种常见的抗生素，具有良好的组织渗透性，低毒性和良好的风险特征，它代表了未来脑膜瘤PDT试验的临床有趣方法。

著录项

  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2014年第1期|1-6|共6页
  • 作者

    Jan Frederick Cornelius; Philipp Joerg Slotty; Mustafa El Khatib; Alexander Giannakis; Brigitte Senger; Hans Jakob Steiger;

  • 作者单位

    Neurochirurgische Klinik, Universitaetsklinikum Dusseldorf, Heinrich-Heine-Universitaet, Moorenstrasse 5, 40229 Duesseldorf, Germany,Neurosurgical Clinic, Heinrich-Heine-Universitaet Duesseldorf, 40225 Duesseldorf, Germany;

    Neurochirurgische Klinik, Universitaetsklinikum Dusseldorf, Heinrich-Heine-Universitaet, Moorenstrasse 5, 40229 Duesseldorf, Germany;

    Neurochirurgische Klinik, Universitaetsklinikum Dusseldorf, Heinrich-Heine-Universitaet, Moorenstrasse 5, 40229 Duesseldorf, Germany;

    Neurochirurgische Klinik, Universitaetsklinikum Dusseldorf, Heinrich-Heine-Universitaet, Moorenstrasse 5, 40229 Duesseldorf, Germany;

    Neurochirurgische Klinik, Universitaetsklinikum Dusseldorf, Heinrich-Heine-Universitaet, Moorenstrasse 5, 40229 Duesseldorf, Germany;

    Neurochirurgische Klinik, Universitaetsklinikum Dusseldorf, Heinrich-Heine-Universitaet, Moorenstrasse 5, 40229 Duesseldorf, Germany;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    5-ALA; Cancer therapy; Celt culture; Meningioma model; Photodynamic therapy;

    机译：5-ALA;癌症治疗;凯尔特文化脑膜瘤模型;光动力疗法;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号