• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Photodynamic effect of Radachlorin on nerve and glial cells
【24h】

Photodynamic effect of Radachlorin on nerve and glial cells

机译：雷达霉素对神经和神经胶质细胞的光动力作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Radachlorin, a chlorine-derived photosensitizer, is used currently in photodynamic therapy (PDT) of skin cancer. In this work we studied Radachlorin-PDT effect on peripheral nerve and glial cells that are damaged along with tumor tissue. Methods: We used simple model objects - a crayfish stretch receptor that consists of a single sensory neuron surrounded by glial cells and crayfish nerve cord consisting of nerve fibers and ganglia. Radachlorin absorption and emission spectra were registered using spectrophotometer and spectrofluorimeter. Radachlorin accumulation and intracellular localization were studied using the fluorescence microscope. Necrotic and apoptotic cells were visualized using propidium iodide and Hoechst 33342. Neuronal activity was registered using standard electrophysiological methods. Results: Radachlorin absorption spectrum in the physiological van Harreveld saline (pH 7.3) contained maximums at 420 and 654 nm. Its fluorescence band 620-700 nm had a maximum at 664 nm. In the crayfish stretch receptor Radachlorin localized predominantly to the glial envelope and penetrated slightly into the neuron body and axon. Radachlorin rapidly accumulated in the crayfish nerve cord tissue within 30 min. Its elimination in the dye-free solution occurred slower: 11% loss for 2 h. Radachlorin-PDT inactivated the neuron and induced necrosis of neurons and glial cells and glial apoptosis at concentrations as low as 10~(-10)-10~(-9)M. Conclusions: Radachlorin rapidly accumulates in the nervous tissue, mainly in glial cells, and demonstrates very high photodynamic efficacy that characterize it as a promising photosensitizer.
机译：背景：Radachlorin是一种氯衍生的光敏剂，目前用于皮肤癌的光动力疗法（PDT）。在这项工作中，我们研究了Radachlorin-PDT对与肿瘤组织一起受损的周围神经和神经胶质细胞的作用。方法：我们使用了简单的模型对象-小龙虾拉伸受体，它由被胶质细胞和小龙虾神经线包围的单个感觉神经元组成，小龙虾神经线由神经纤维和神经节组成。使用分光光度计和分光荧光计记录了拉达霉素的吸收和发射光谱。使用荧光显微镜研究了拉达霉素的积累和细胞内定位。使用碘化丙锭和Hoechst 33342观察坏死和凋亡细胞。使用标准电生理方法记录神经元活性。结果：生理范哈雷维尔德盐水（pH 7.3）中的Radachlorin吸收光谱在420和654 nm处具有最大值。其620-700nm的荧光带在664nm处具有最大值。在小龙虾的拉伸受体中，Radachlorin主要定位于神经胶质包膜，并轻微渗入神经元体和轴突。 Radachlorin在30分钟内迅速积聚在小龙虾的神经索组织中。在无染料溶液中的消除速度较慢：2小时损失11％。 Radachlorin-PDT在低至10〜（-10）-10〜（-9）M的浓度下能使神经元失活，并诱导神经元和神经胶质细胞坏死和神经胶质细胞凋亡。结论：瑞达霉素在神经组织中迅速积累，主要在神经胶质细胞中积累，并显示出很高的光动力学功效，这使其成为有前途的光敏剂。

著录项

  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2014年第3期|357-364|共8页
  • 作者

    M.A. Neginskaya; E.V. Berezhnaya; M.V. Rudkovskii; S.V. Demyanenko; A.B. Uzdensky;

  • 作者单位

    A.B. Kogan Research Institute for Neurocybernetics, Southern Federal University, Rostov-on-Don 344090, Russia;

    A.B. Kogan Research Institute for Neurocybernetics, Southern Federal University, Rostov-on-Don 344090, Russia;

    Department of Biophysics and Biocybernetics, Southern Federal University, Rostov-on-Don 3440290, Russia;

    Department of Biophysics and Biocybernetics, Southern Federal University, Rostov-on-Don 3440290, Russia;

    Department of Biophysics and Biocybernetics, Southern Federal University, Rostov-on-Don 3440290, Russia,A.B. Kogan Research Institute for Neurocybernetics, 194/1 Stachky Ave., NII NK, Rostov-on-Don 344090, Russia;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Photodynamic therapy; Radachlorin; Neuron; Glia; Necrosis; Apoptosis;

    机译：光动力疗法;拉达霉素;神经元;胶质细胞坏死;细胞凋亡;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号