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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Influence of ultrasonic activation on photodynamic therapy over root canal system infected with Enterococcus faecalis - an in vitro study
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Influence of ultrasonic activation on photodynamic therapy over root canal system infected with Enterococcus faecalis - an in vitro study

机译：超声激活对粪肠球菌感染根管系统光动力疗法的影响-体外研究

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Aim: The purpose of this study was to evaluate, in vitro, the influence of ultrasonic activation on photodynamic therapy over root canal system infected with Enterococcus faecalis. Material and methods: The root canals of 50 single-rooted human extracted teeth were enlarged up to a file 60, autoclaved, inoculated with Enterococcus faecalis and incubated for 30 days. The samples were divided into five groups (n = 10) according to the protocol of decontamination: G1 (control group) - no procedure was performed; G2 - photosensitizer (0.01% methylene blue); G3 - ultrasonic activation of photosensitizer (0.01% methylene blue); G4 - photodynamic therapy with no ultrasonic activation; and G5 - photodynamic therapy with ultrasonic activation. Microbiological tests (CFU counting) and scanning electron microscopy (SEM) were performed to evaluate and illustrate, respectively, the effectiveness of proposed treatments. Data were subjected to one-way ANOVA followed by post hoc Tukey test (a = 0.05). Results: The microbiological test demonstrated that G5 (photodynamic therapy with ultrasonic activation) showed the lowest mean contamination (3.17 log CFU/mL), which was statistically different from all other groups (p ＜ 0.05). G4 (photodynamic therapy) showed a mean of contamination of 3.60 log CFU/mL, which was statistically different from groups 1, 2 and 3 (p＜0.05). Conclusion: The use of ultrasonic activation on photodynamic therapy improved its potential for decontamination, resulting in the higher elimination Enterococcus faecalis from the root canal space.
机译：目的：本研究的目的是在体外评估超声激活对粪便肠球菌感染的根管系统光动力疗法的影响。材料和方法：将50只单根人拔牙的根管扩大至锉60，高压灭菌，用粪肠球菌接种，并孵育30天。根据去污方案将样品分为五组（n = 10）：G1（对照组）-不执行任何程序； G2-光敏剂（0.01％亚甲蓝）； G3-光敏剂的超声活化（0.01％亚甲蓝）； G4-没有超声激活的光动力疗法；和G5-超声激活的光动力疗法。进行了微生物学测试（CFU计数）和扫描电子显微镜（SEM）以分别评估和说明所提出治疗的有效性。对数据进行单向方差分析，然后进行事后Tukey测试（a = 0.05）。结果：微生物测试表明，G5（超声激活光动力疗法）显示出最低的平均污染（3.17 log CFU / mL），与所有其他组相比在统计学上均存在差异（p ＜0.05）。 G4（光动力疗法）显示平均污染为3.60 log CFU / mL，与第1、2和3组相比有统计学差异（p ＜0.05）。结论：在光动力疗法上使用超声波激活提高了其去污潜能，从而从根管腔中消除了更高的粪肠球菌。

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