• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Cyanines in photodynamic reaction assisted by reversible electroporation-in vitro study on human breast carcinoma cells
【24h】

Cyanines in photodynamic reaction assisted by reversible electroporation-in vitro study on human breast carcinoma cells

机译：可逆电穿孔辅助光合作用中的花菁对人乳腺癌细胞的体外研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Ineffective drug delivery is a vast problem of anticancer therapies. The aim of this study was to investigate the possibility of enhancement of cyanines transport through the cell membrane by electroporation and to evaluate a photodynamic activity of these compounds. Methods: We evaluated in vitro the effectiveness of photodynamic reaction with cyanines on breast adenocarcinoma cells (MCF-7/WT) and normal Chinese hamster ovary cells (CHO) lacking voltage-dependention channels, alone and combined with electropermeabilization. Among six cyanines tested, two compounds could be indicated as good therapeutic candidates: IR-775 and IR-786. Cellular effects were assessed with MTT assay reporting cell mitochondrial activity and with SRB assay based on the measurement of cellular protein content. Cyanines localization was observed with confocal microscope. Results: Photodynamic reaction of MCF-7/WT cells with IR-775 and IR-786 did not result in cellular dysfunction. Electric field intensities and pulse duration, non-toxic for cells, significantly increased photocytotoxicity of the cyanines after electropermeabilization with IR-775 and IR-786. Much shorter exposure times were efficient for cyanines in photodynamic reaction assisted by electroporation (10min instead of 24 h). Conclusions: Our results indicate that electroporation of cancerous cells in the presence of cyanine dyes could increase the uptake of the photosensitizer, which correlates with a higher cytotoxicity in the breast adenocarcinoma cell line. Electroporation may be an attractive delivery system for photosensitizers in photodynamic therapy, enabling application of new compounds and reduction of drug dose and exposure time.
机译：背景：无效的药物输送是抗癌治疗的一个巨大问题。这项研究的目的是研究通过电穿孔增强花青素通过细胞膜转运的可能性，并评估这些化合物的光动力活性。方法：我们在体外评估了花青素对乳腺腺癌细胞（MCF-7 / WT）和缺乏电压依赖性通道的正常中国仓鼠卵巢细胞（CHO）的光动力反应的有效性，该作用单独且与电透化结合使用。在测试的六种花菁中，两种化合物可被指示为良好的治疗候选物：IR-775和IR-786。通过报告细胞线粒体活性的MTT分析和基于细胞蛋白质含量测量的SRB分析评估细胞效应。用共聚焦显微镜观察花青的定位。结果：MCF-7 / WT细胞与IR-775和IR-786的光动力反应未导致细胞功能障碍。对细胞无毒的电场强度和脉冲持续时间，在用IR-775和IR-786进行电透化后，大大提高了花菁的光细胞毒性。花青素在电穿孔辅助下的光动力反应中，曝光时间要短得多（10分钟而不是24小时）。结论：我们的结果表明在花菁染料存在下癌细胞的电穿孔可以增加光敏剂的摄取，这与乳腺癌细胞系中较高的细胞毒性有关。电穿孔对于光动力疗法中的光敏剂而言可能是有吸引力的递送系统，从而能够应用新化合物并减少药物剂量和暴露时间。

著录项

  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2013年第4期|490-502|共13页
  • 作者

    Joanna Wezgowiec; Malgorzata Kotulska; Jolanta Saczko; Maria B. Derylo; Justin Teissie; Marie-Pierre Rols; Julie Orio; Arnold Garbiec; Julita Kulbacka;

  • 作者单位

    Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, WybrzezeWyspianskiego 27, 50-370 Wroclaw, Poland;

    Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, WybrzezeWyspianskiego 27, 50-370 Wroclaw, Poland;

    Department of Medical Biochemistry, Wroclaw Medical University, ul. Chalubinskiego 10, 50-368 Wroclaw,Poland;

    Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, WybrzezeWyspianskiego 27, 50-370 Wroclaw, Poland;

    CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, F-31077Toulouse, France,Universite de Toulouse, UPS, IPBS, F-31077 Toulouse, France;

    CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, F-31077Toulouse, France,Universite de Toulouse, UPS, IPBS, F-31077 Toulouse, France;

    CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, F-31077Toulouse, France,Universite de Toulouse, UPS, IPBS, F-31077 Toulouse, France;

    Department of Animal Developmental Biology, Zoological Institute, University of Wroclaw, ul.Sienkiewicza 21, 50-335 Wroclaw, Poland;

    Department of Medical Biochemistry, Wroclaw Medical University, ul. Chalubinskiego 10, 50-368 Wroclaw,Poland;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Photodynamic reaction; Electropermeabi lization; Electroporation; Drug delivery; Cyanine; Breast cancer cells;

    机译：光动力反应;电渗透性;电穿孔;药物输送;花青;乳腺癌细胞;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号