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Electroporation enhances antimicrobial photodynamic therapy mediated by the hydrophobic photosensitizer, hypericin

机译：电穿孔增强了疏水性光敏剂金丝桃素介导的抗微生物光动力疗法

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摘要

The effective transport of photosensitizers (PS) across the membrane and the intracellular accumulation of PS are the most crucial elements in antimicrobial photodynamic therapy (aPDT). However, due to the morphological complexity of Gram-negative bacteria the penetration of PS is limited, especially hydrophobic PS. Electroporation (EP) could increase the effectiveness of aPDT, by promoting the formation of transient pores that enhance the permeability of the bacterial membrane to PS. In this study we evaluated the combination of aPDT mediated by the hydrophobic PS, hypericin and EP (aPDT/EP) against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. These bacteria were exposed to light (590 nm) in the presence of hypericin (4 μM), following electroporation. The results showed that aPDT/EP inactivated 3.67 logs more E. coli and 2.65 logs more S. aureus than aPDT alone. Based on these results we suaaest that EP can potentiate the aPDT effect.
机译：有效的光敏剂（PS）跨膜运输和PS的细胞内积累是抗菌光动力疗法（aPDT）中最关键的要素。然而，由于革兰氏阴性细菌的形态复杂性，PS的渗透受到限制，特别是疏水性PS。电穿孔（EP）可以通过促进瞬态孔的形成来提高aPDT的有效性，该孔可增强细菌膜对PS的渗透性。在这项研究中，我们评估了疏水性PS，金丝桃素和EP（aPDT / EP）介导的针对抗革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌的aPDT的组合。电穿孔后，在金丝桃素（4μM）存在下，将这些细菌暴露于光（590 nm）中。结果表明，与单独的aPDT相比，aPDT / EP灭活的大肠杆菌和金黄色葡萄球菌的灭活率分别为3.67和2.65。根据这些结果，我们认为EP可以增强aPDT效应。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2013年第4期|647-650|共4页
  • 作者

    Wanessa de Cassia Martins Antunes de Melo; Alexander N. Lee; Janice Rodrigues Perussi; Michael R. Hamblin;

  • 作者单位

    Programa de Pos-Craduagao Interunidades em Bioengenharia - EESC/FMRP/IQSC,Universidade de Sao Paulo, Sao Carlos, SP, Brazil,Wetlman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA,Department of Dermatology, Harvard Medical School, Boston, MA, USA;

    Wetlman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA,John Jay High School, Cross River, NY, USA;

    Programa de Pos-Craduagao Interunidades em Bioengenharia - EESC/FMRP/IQSC,Universidade de Sao Paulo, Sao Carlos, SP, Brazil,Instituto de Quimica de Sao Carlos, Universidade de Sao Paulo, Sao Carlos, SP, Brazil;

    Wetlman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA,Department of Dermatology, Harvard Medical School, Boston, MA, USA,Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, USA;

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  • 正文语种 eng
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  • 关键词

    Antimicrobial photodynamic therapy; Hypericin; Electroporation; E. coli; S. aureus;

    机译：抗菌光动力疗法;金丝桃素;电穿孔;大肠杆菌;金黄色葡萄球菌;

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