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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Increased number of fractionated irradiation sessions does not improve the cellular response to methyl aminolevulinate-mediated photodynamic therapy
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Increased number of fractionated irradiation sessions does not improve the cellular response to methyl aminolevulinate-mediated photodynamic therapy

机译：分段照射次数增加并不能改善细胞对氨基乙酰丙酸甲酯介导的光动力疗法的反应

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Background: In the search to improve the efficacy of photodynamic therapy different approaches have been investigated until now. One of these approaches is the use of double fractionated irradiation schemes. This study aims to investigate the effect of increasing the numbers of fractionated irradiation sessions in methyl aminolevulinate-mediated photodynamic therapy on dysplastic oral keratinocytes by monitoring the changes in optical properties of cell culture. Methods: Forty cell cultures were divided into four experimental groups (single, double and triple fractionated irradiation scheme and control). Cells were incubated with 1 mM methyl aminolevulinate and after 2 h they were exposed to laser radiation (λ = 635 nm, P= 15 mW). The exposure time was 900 s for the single irradiation scheme, 300 s +600 s for the double fractionated irradiation scheme with dark interval between sessions of 30min and 300 s + 300 s +300 s for the triple fractionated irradiation scheme with two dark intervals between sessions of 30min. Two optical parameters (refractive index and extinction coefficient) were calculated from diffuse reflectance spectra of cell cultures using the Kramers-Kronig relations. Results: The results demonstrate that the use of double fractionated irradiation scheme led to changes in optical parameters of cell cultures more important than the use of the single and triple fractionated irradiation schemes in photodynamic therapy. Conclusions: In conclusion, increasing too much the number of irradiation sessions cannot lead to an improved photodynamic effect.
机译：背景技术：为了提高光动力疗法的疗效，迄今为止已经研究了不同的方法。这些方法之一是使用双重分级照射方案。这项研究旨在通过监测细胞培养物光学特性的变化，研究增加氨基乙酰丙酸甲酯介导的光动力治疗中分次照射的次数对发育不良的口腔角质形成细胞的影响。方法：将40种细胞培养物分为四个实验组（单次，两次和三次分级照射方案和对照）。将细胞与1 mM氨基乙酰丙酸甲酯一起孵育，并在2小时后将其暴露于激光辐射（λ= 635 nm，P = 15 mW）。单次照射方案的曝光时间为900 s，双分次照射方案的曝光时间为300 s +600 s，两次照射之间的暗间隔为30分钟；三重分份照射方案的曝光时间为300 s + 300 s +300 s，两次照射之间为暗间隔每节30分钟。使用Kramers-Kronig关系从细胞培养物的漫反射光谱计算出两个光学参数（折射率和消光系数）。结果：结果表明，在光动力疗法中，使用双级照射方案比使用单级和三级照射方案更重要的是改变细胞培养物的光学参数。结论：总而言之，增加过多的照射次数不会导致改善的光动力效应。

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