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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Cutaneous penetration of the topically applied photosensitizer Pc 4 as detected by intravital 2-photon laser scanning microscopy
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Cutaneous penetration of the topically applied photosensitizer Pc 4 as detected by intravital 2-photon laser scanning microscopy

机译：活体2光子激光扫描显微镜检测到的局部应用光敏剂Pc 4的皮肤渗透

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摘要

The fundamental mechanism of photodynamic therapy (PDT)-induced cell death has been characterized, but early critical PDT events in vivo remain incompletely defined. With the recent development in advanced fluorescence imaging modalities, such as intravital 2-photon laser scanning microscopy (2P-LSM), researchers are now able to investigate and visualize biological processes with high resolution in real time. This powerful imaging technology allows deep tissue visualization with single-cell resolution, thus providing dynamic information on the 3-dimensional architectural makeup of the tissue. The main goal of this study was to determine the cutaneous penetration of a topically applied photosensitizer, the silicon phthalocyanine Pc 4, into the skin of live animals and to assess the effective absorption of Pc 4 through the skin barrier. Our 2P-LSM images indicate that Pc 4 penetrates to the epidermal/dermal junction of mouse skin. The data also indicate that the degree of Pc 4 absorption is dose dependent. These findings represent initial steps that may help in improving the clinical utilization of topical Pc 4-PDT. Published by Elsevier B.V.
机译：光动力疗法（PDT）诱导的细胞死亡的基本机制已被表征，但体内早期的关键PDT事件仍未完全确定。随着先进的荧光成像模式（例如活体2光子激光扫描显微镜（2P-LSM））的最新发展，研究人员现在能够实时研究和可视化高分辨率的生物过程。这项强大的成像技术可实现具有单细胞分辨率的深层组织可视化，从而提供有关3维组织结构构成的动态信息。这项研究的主要目的是确定局部应用的光敏剂硅酞菁Pc 4进入活体动物皮肤的穿透力，并评估Pc 4通过皮肤屏障的有效吸收。我们的2P-LSM图像表明Pc 4渗透到小鼠皮肤的表皮/真皮连接处。数据还表明Pc 4吸收程度与剂量有关。这些发现代表了可能有助于改善局部Pc 4-PDT的临床利用率的初始步骤。由Elsevier B.V.发布

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2012年第3期|p.225-231|共7页
  • 作者

    Alex Y. Huang; Jay T. Myers; Deborah Barkauskas; Scott J. Howell; Nancy L. Oleinick; Thomas S. McCormick; Kevin D. Cooper; Elma D. Baron; Minh Lam;

  • 作者单位

    Division of Pediatric HematologylOncology, Department of Pediatrics, Case Western Reserve University/ University Hospitals Case Medical Center, Cleveland, OH, United States, Case Comprehensive Cancer Center, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States;

    Division of Pediatric HematologylOncology, Department of Pediatrics, Case Western Reserve University/ University Hospitals Case Medical Center, Cleveland, OH, United States;

    Division of Pediatric HematologylOncology, Department of Pediatrics, Case Western Reserve University/ University Hospitals Case Medical Center, Cleveland, OH, United States;

    Case Visual Sciences Research Center, Case Western Reserve University/University Hospitals Case Medical Center, Cleveland, OH, United States;

    Case Comprehensive Cancer Center, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States,Department of Radiation Oncology, Case Western Reserve University/University Hospitals Case Medical Center, Cleveland, OH, United States;

    Department of Dermatology, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States,Case Skin Diseases Research Center, Case Western Reserve University/University Hospitals Case Medical Center, Cleveland, OH, United States;

    Case Comprehensive Cancer Center, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States,Department of Dermatology, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States,Case Skin Diseases Research Center, Case Western Reserve University/University Hospitals Case Medical Center, Cleveland, OH, United States, Louis-Stokes VA Medical Center, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States;

    Case Comprehensive Cancer Center, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States,Department of Dermatology, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States,Case Skin Diseases Research Center, Case Western Reserve University/University Hospitals Case Medical Center, Cleveland, OH, United States, Louis-Stokes VA Medical Center, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States;

    Case Comprehensive Cancer Center, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States,Department of Dermatology, Case Western Reserve UniversityI University Hospitals Case Medical Center, Cleveland, OH, United States, Department of Dermatology, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, United States;

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  • 正文语种 eng
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  • 关键词

    Pc 4; photodynamic therapy (PDT); intravital microscopy; 2-photon laser scanning microscopy (2P-LSM);

    机译：PC 4;光动力疗法（PDT）;活体显微镜检查2光子激光扫描显微镜（2P-LSM）;

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