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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Photodiagnosis and photodynamic therapy of endometriotic epithelial cells using 5-aminolevulinic acid and steroids
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Photodiagnosis and photodynamic therapy of endometriotic epithelial cells using 5-aminolevulinic acid and steroids

机译：5-氨基乙酰丙酸和类固醇对子宫内膜异位上皮细胞的光诊断和光动力治疗

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Background: The photodynamic diagnosis and therapy represent relatively new methods used, i.a., in the detection of some preneoplastic and neoplastic conditions. They are based on selective accumulation of photosensitizers in the altered cells, which can be identified by fluorescence of the sensitizers and, using light of an appropriate wavelength, can be eliminated. Currently, investigations continue on application of the methods in diagnosis and therapy of endometriosis, one of the most prevalent causes of a reduced fertility in women. Methods: In this study protoporphyrin IX, a photosensitizer derived from 5-aminolevulinic acid, was used to locate and destroy endometrial epithelium. Material for the investigations involved primary epithelial cells, isolated from 15 normal endometria and 15 ovarian endometriotic epithelia. Taking into account the cyclical hormonal alterations, which affect endometrial cells in individual phases of the menstrual cycle, experiments were conducted on accumulation of the photosensitizer and photodestruction of the cells preceded by their hormonal stimulation (17β-estradiol and progesterone). Results and conclusion: It was found that following 48 h stimulation with 17β-estradiol and/or progesterone a significantly augmented synthesis of protoporphyrin IX can be obtained in cells of endometrial epithelium as compared to the normal epithelium. Moreover, the endometriotic epithelial cells were most effectively eliminated following 48 h prestimulation with progesteron alone. The obtained result permits to assume that photodynamic diagnosis and photodynamic therapy of endometrial epithelium should be performed in the secretory phase of endometrium in order to optimise their results.
机译：背景：光动力诊断和治疗代表了相对较新的方法，即用于检测某些肿瘤前和肿瘤状态。它们基于光敏剂在改变的细胞中的选择性积累，这可以通过敏化剂的荧光来鉴定，并且可以使用适当波长的光来消除。目前，有关该方法在子宫内膜异位症的诊断和治疗中的应用的研究仍在继续，子宫内膜异位症是女性生育力降低的最普遍原因之一。方法：在这项研究中，原卟啉IX（一种源自5-氨基乙酰丙酸的光敏剂）用于定位和破坏子宫内膜上皮。用于研究的材料涉及从15例正常子宫内膜和15例卵巢子宫内膜异位上皮细胞分离的原代上皮细胞。考虑到周期性激素的变化会影响月经周期各个阶段的子宫内膜细胞，因此进行了光敏剂的积累和光破坏，然后再进行激素刺激（17β-雌二醇和孕酮）的实验。结果和结论：发现在用17β-雌二醇和/或孕酮刺激48小时后，与正常上皮相比，子宫内膜上皮细胞中原卟啉IX的合成明显增加。此外，仅用孕激素刺激48小时后，子宫内膜异位上皮细胞最有效地被清除。获得的结果允许假定子宫内膜上皮的光动力诊断和光动力治疗应在子宫内膜的分泌期进行，以优化其结果。

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