首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Differences in sensitivity to HMME-mediated photodynamic therapy between EBV+ C666-1 and EBV- CNE2 cells
【24h】

Differences in sensitivity to HMME-mediated photodynamic therapy between EBV+ C666-1 and EBV- CNE2 cells

机译:EBV + C666-1和EBV-CNE2细胞对HMME介导的光动力疗法的敏感性差异

获取原文
获取原文并翻译 | 示例
       

摘要

Objectives: The aim of this study was to compare the sensitivity of hematoporphyrin monomethyl ether (HMME)-mediated PDT in the Epstein-Barr virus (EBV) positive and negative human nasopharyngeal carcinoma (NPC) cells.rnMethods and materials: The intracellular uptake of HMME in C666-1 (EBV+) and CNE2 (EBV-) cells was quantitatively determined after incubating with different concentration of HMME. The subcellular localization of HMME was examined under a laser scanning fluorescence microscopy. The survival rate of C666-1 and CNE2 cells after HMME PDT was evaluated with clonogenic assay. Results: The EBV- CNE2 cells showed higher intracellular uptake of HMME and lower survival rate than EBV+ C666-1 cells. There was no significant difference in terms of subcellular localization of HMME between C666-1 and CNE2 cells.rnConclusion: The sensitivity of EBV- CNE2 cells to HMME-mediated PDT was significantly higher than that of EBV+ C666-1 cells. The intracellular uptake of HMME plays a key role in determining the PDT sensitivity.
机译:目的:本研究旨在比较血卟啉单甲醚(HMME)介导的PDT在爱泼斯坦-巴尔病毒(EBV)阳性和阴性人鼻咽癌(NPC)细胞中的敏感性。方法和材料:细胞内摄取与不同浓度的HMME孵育后,定量测定C666-1(EBV +)和CNE2(EBV-)细胞中的HMME。在激光扫描荧光显微镜下检查HMME的亚细胞定位。用克隆形成试验评估HMME PDT后C666-1和CNE2细胞的存活率。结果:EBV-CNE2细胞比EBV + C666-1细胞具有更高的HMME细胞内摄取率和较低的存活率。结论:EBV-CNE2细胞对HMME介导的PDT的敏感性明显高于EBV + C666-1细胞。对C666-1和CNE2细胞的HMME亚细胞定位无明显差异。 HMME的细胞内摄取在确定PDT敏感性中起关键作用。

著录项

  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2010年第3期|p.204-209|共6页
  • 作者单位

    Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fujian, Fuzhou 350007, China;

    rnKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fujian, Fuzhou 350007, China;

    rnKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fujian, Fuzhou 350007, China;

    rnKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fujian, Fuzhou 350007, China;

    Department of Radiation Oncology, University of Colorado Denver, Denver, CO, USA;

    rnKey Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fujian, Fuzhou 350007, China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    nasopharyngeal carcinoma; photodynamic therapy; hematoporphyrin monomethyl ether; intracellular uptake; subcellular localization;

    机译:鼻咽癌光动力疗法;血卟啉单甲醚;细胞内摄取;亚细胞定位;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号