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首页> 外文期刊>Photodiagnosis and photodynamics therapy >Clinical and immunohistochemical assessment of vulval intraepithelial neoplasia following photodynamic therapy using a novel bioadhesive patch-type system loaded with 5-aminolevulinic acid
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Clinical and immunohistochemical assessment of vulval intraepithelial neoplasia following photodynamic therapy using a novel bioadhesive patch-type system loaded with 5-aminolevulinic acid

机译：使用新型生物粘附贴剂型系统加载5-氨基乙酰丙酸进行光动力治疗后外阴上皮内瘤变的临床和免疫组化评估

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摘要

Background: The work in this study appraised photodynamic treatment (PDT) as a treatment method for vulval intraepithelial neoplasia (VIN) using a novel bioadhesive patch to deliver aminolevulinic acid. An analysis of changes in expression of apoptotic and cell cycle proteins (p53, p21, Mdm2, Blc-2, Bax, Ki-67) in response to PDT was evaluated. Methods: PDT was performed using non-laser light, either as a one or two-cycle treatment, with clinical and pathological assessment following after 6 weeks. Twenty-three patients with 25 VIN lesions underwent 49 cycles of PDT. Patches were designed to conform to uneven vulval skin and contained 38 mg cm~(-2) aminolevulinic acid. Assessment was carried out at 6 weeks post-treatment. Patient-based treatment assessment, along with clinical and pathological changes, were monitored. Immunohistochemical staining was used to elucidate a possible biomolecular basis for induced cellular changes.rnResults: Most patients (52%) reported a symptomatic response, with normal pathology restored in 38% of lesions. The patch was easy to apply and remove, causing minimal discomfort. Fluorescence inspection confirmed protoporphyrin accumulation. Pain during implementation of PDT was problematic, necessitating some form of local analgesia. Changes in expression of cell cycle and apoptotic-related proteins suggested involvement of apoptotic pathways. Down regulation of p21 and inverse changes in Bcl-2 and Bax were key findings.
机译：背景：这项研究的工作评价了光动力治疗（PDT）作为一种使用新型生物粘附贴剂递送氨基乙酰丙酸的外阴上皮内瘤变（VIN）的治疗方法。评估了针对PDT的凋亡和细胞周期蛋白（p53，p21，Mdm2，Blc-2，Bax，Ki-67）表达变化的分析。方法：PDT使用非激光光进行一轮或两轮治疗，并在6周后进行临床和病理评估。有25个VIN病变的23例患者接受了49个PDT周期。设计贴剂以适应不规则的外阴皮肤并包含38 mg cm〜（-2）氨基乙酰丙酸。在治疗后6周进行评估。监测基于患者的治疗评估以及临床和病理变化。结果：大多数患者（52％）表现出症状反应，38％的病变恢复了正常病理，因此采用了免疫组织化学染色来阐明诱导的细胞变化的可能的生物分子基础。该贴剂易于施用和去除，从而将不适感降至最低。荧光检查证实原卟啉积累。 PDT实施过程中的疼痛是有问题的，需要某种形式的局部镇痛。细胞周期和凋亡相关蛋白表达的变化提示凋亡途径的参与。主要发现是p21的下调以及Bcl-2和Bax的逆变化。

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  • 来源
    《Photodiagnosis and photodynamics therapy》 |2009年第1期|28-40|共13页
  • 作者

    A. Zawislak; R.F. Donnelly; W.G. McCluggage; J.H. Price; H.R. McClelland; A.D. Woolfson; S. Dobbs; P. Maxwell; P.A. McCarron;

  • 作者单位

    Department of Gynaecology, Belfast City Hospital Trust, Lisburn Road, Belfast BT9 7AB, UK;

    School of Pharmacy, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UK;

    Department of Pathology, Royal Group of Hospitals Trust, Grosvenor Road, Belfast BT12 6BA, UK;

    Department of Gynaecology, Belfast City Hospital Trust, Lisburn Road, Belfast BT9 7AB, UK;

    Department of Gynaecology, Belfast City Hospital Trust, Lisburn Road, Belfast BT9 7AB, UK;

    School of Pharmacy, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UK;

    Department of Gynaecology, Belfast City Hospital Trust, Lisburn Road, Belfast BT9 7AB, UK;

    Department of Pathology, Royal Group of Hospitals Trust, Grosvenor Road, Belfast BT12 6BA, UK;

    Department of Pharmacy and Pharmaceutical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, UK;

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  • 关键词

    vulval intraepithelial neoplasia; photodynamic therapy; aminolevulinfc acid; bioadhesive; patch;

    机译：外阴上皮内瘤变;光动力疗法;氨基乙酰丙酸生物粘合剂补丁;

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