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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo-induced biomodulation
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Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo-induced biomodulation

机译：可见光通过光诱导生物调节诱导小鼠骨髓间充质干细胞成脂分化

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摘要

Background: Bone marrow mesenchymal stem cells (BM-MSCs) are undifferentiated cells that can proliferate and differentiate into specialized cells for tissue self-repair. Low-level laser (LLL) can induce biomodulatory effects such as cellular proliferation, differentiation, and migration. We investigated the biomodulatory effects of the photoactive compound chloroaluminum phthalocyanine nanoemulsion (AlClPc/NE) on the adipogenic differentiation of BM-MSCs, when combined with LLL (AlClPc/NE-LLL).Methods: The BM-MSCs used in this work were isolated from green fluorescent protein-positive (GFP(+)) C57BL6 mice. Cells were first treated with AlClPc/NE, a well-designed photoactive nano-drug and were then subjected to in vitro expansion, morphological and immunophenotypic characterization, and cellular cytotoxicity analysis. Subsequently, BM-MSCs were induced to differentiate into adipocytes by photo-induced biomodulation with AlClPc/NE-LLL.Results: Our results showed that the isolated cell population was consistent with murine BM-MSCs. The cellular cytotoxicity analysis revealed that the optimal nanoemulsion dose to induce BM-MSC biomodulation was 5.0 mu mol/L. Twenty-four hours following treatment with AlClPc/NE, BM-MSC were subjected to visible light irradiation of 20 mJ/cm(2) at 670 nm. Six days after photo-induced biomodulation, cells maintained high GFP expression level, and expressed detectable mRNA levels of adipogenic genes (lipoprotein lipase and PPAR gamma); formation of lipid vacuoles was observed, and the cells did not show any tumorigenic potential in vivo.Conclusions: Our results indicated that photo-induced biomodulation via visible light using AlClPc/NE and LLL can induce adipogenic differentiation of murine BM-MSCs. Therefore, cell therapy with BM-MSCs and photoinduced biomodulation may contribute to the development of new therapeutic strategies that are faster and more effective than traditional methods to trigger MSC differentiation.
机译：背景：骨髓间充质干细胞（BM-MSC）是未分化的细胞，可以增殖并分化为用于组织自我修复的专门细胞。低能激光（LLL）可以诱导生物调节作用，例如细胞增殖，分化和迁移。我们研究了光活性化合物氯铝酞菁纳米乳液（AlClPc / NE）与LL-LLL（AlClPc / NE-LLL）结合时对BM-MSCs成脂分化的生物调节作用。方法：分离用于这项工作的BM-MSCs来自绿色荧光蛋白阳性（GFP（+））C57BL6小鼠。首先用精心设计的光敏纳米药物AlClPc / NE处理细胞，然后进行体外扩增，形态学和免疫表型鉴定以及细胞毒性分析。随后，通过AlClPc / NE-LLL的光诱导生物调节，诱导BM-MSC分化为脂肪细胞。结果：我们的结果表明，分离的细胞群与鼠BM-MSC一致。细胞毒性分析表明，诱导BM-MSC生物调节的最佳纳米乳剂量为5.0μmol/ L。用AlClPc / NE处理后二十四小时，BM-MSC在670 nm处受到20 mJ / cm（2）的可见光照射。光诱导生物调节后六天，细胞保持高GFP表达水平，并表达可检测的脂肪生成基因（脂蛋白脂肪酶和PPARγ）mRNA水平。结论：我们的结果表明，使用AlClPc / NE和LLL通过可见光进行光诱导的生物调节可诱导鼠BM-MSC的成脂分化。因此，用BM-MSC进行细胞治疗和光诱导生物调节可能有助于开发新的治疗策略，该策略比触发MSC分化的传统方法更快，更有效。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2019年第3期|119-127|共9页
  • 作者

    Castilho-Fernandes Andrielle; Lopes Tadla G.; Ferreira Fernando U.; Rezende Nayara; Silva Valeria F.; Primo Fernando L.; Fontes Aparecida Maria; Ribeiro-Silva Alfredo; Tedesco Antonio Claudio;

  • 作者单位

    Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem,Photobiol & Photomed Res Grp, BR-14040901 Ribeirao Preto, Brazil;

    Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem,Photobiol & Photomed Res Grp, BR-14040901 Ribeirao Preto, Brazil;

    Univ Sao Paulo, Ctr Cell Therapy, Med Sch, Natl Inst Sci & Technol Stem Cell & Cell Therapy, BR-14051140 Ribeirao Preto, Brazil|Univ Sao Paulo, Reg Blood Ctr, Med Sch, Natl Inst Sci & Technol Stem Cell & Cell Therapy, BR-14051140 Ribeirao Preto, Brazil;

    Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem,Photobiol & Photomed Res Grp, BR-14040901 Ribeirao Preto, Brazil;

    Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem,Photobiol & Photomed Res Grp, BR-14040901 Ribeirao Preto, Brazil|Univ Sao Paulo, Ctr Cell Therapy, Med Sch, Natl Inst Sci & Technol Stem Cell & Cell Therapy, BR-14051140 Ribeirao Preto, Brazil|Univ Sao Paulo, Reg Blood Ctr, Med Sch, Natl Inst Sci & Technol Stem Cell & Cell Therapy, BR-14051140 Ribeirao Preto, Brazil;

    Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem,Photobiol & Photomed Res Grp, BR-14040901 Ribeirao Preto, Brazil|Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara SP 14800-903, BR-14800903 Araraquara, SP, Brazil;

    Univ Sao Paulo, Med Sch, Dept Genet, BR-14051140 Ribeirao Preto, Brazil;

    Univ Sao Paulo, Med Sch, Dept Pathol, BR-14049900 Ribeirao Preto, Brazil;

    Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem,Photobiol & Photomed Res Grp, BR-14040901 Ribeirao Preto, Brazil;

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  • 关键词

    Bone marrow-mesenchymal stem cells (BM-MSC); Photo induced biomodulation; Low level laser (LLL); Chloroaluminum phthalocyanine (AlClPc); Adipogenic differentiation;

    机译：骨髓间充质干细胞（BM-MSC）;光诱导生物调制;低水平激光（LLL）;氯铝酞菁（AlClPc）;成脂分化;

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