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Effect of photodynamic antimicrobial chemotherapy on Candida albicans in the presence of glucose

机译：葡萄糖存在下光动力抗菌化疗对白色念珠菌的影响

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Background: Candida albicans is an opportunistic commensal microorganism, often associated with severe infections in immunosuppressed individuals. C. albicans has hexose transporters that may favor the intracellular accumulation of photosensitizer (PS). the aims of this study were to investigate the influence of glucose load on photodynamic antimicrobial chemotherapy (PACT); and the role that membrane transport system plays on this therapy in the presence of glucose.Material and methods: Strains of C. albicans were selected: ATCC 10231, YEM 12, YEM 13, YEM 14 and YEM 15. All strains were grown aerobically on Sabouraud agar and incubated at 30 degrees C for 24 h. The strains were treated with and without glucose, and divided into Control (no treatment), LED light (660 nm, 166 mW/cm(2) ), Photosensitizer (100 mu M methylene blue) and PACT at 1, 3 and 6 min of irradiation groups. The colony forming units were counted and data submitted to statistical analysis (ANOVA) and Tukey's test. The concentration of methylene blue (MB) outside the yeast was measured by fluorescence spectroscopy.Results: PACT inactivate C. albicans and the presence of glucose did not affect the killing effect for most strains. Only YEM12 was partially affected by its presence. Regarding efflux systems, ABC overexpressing strain showed a protective effect on the yeast cells. We observed that yeast with overexpression of major facilitator superfamily (MFS) membrane pore tended to accumulate more MB in its cytoplasm, whereas strains that overexpressed ABC pumps (ATP-binding cassette transporters) tended to decrease MB uptake and survive the photodynamic challenge.Conclusion: Presence of glucose showed a small effect on PACT. The accumulation of MB on yeast induces more photodynamic inactivation; however, the photodynamic efficacy depends on the type and characteristics of the microbial strain.
机译：背景：白色念珠菌是一种机会性共生微生物，通常与免疫抑制个体的严重感染有关。白色念珠菌具有己糖转运蛋白，可能促进光敏剂（PS）在细胞内的积累。本研究的目的是研究葡萄糖负荷对光动力抗菌化学疗法（PACT）的影响。材料和方法：选择白色念珠菌菌株：ATCC 10231，YEM 12，YEM 13，YEM 14和YEM15。所有菌株均在有氧条件下生长Sabouraud琼脂，在30摄氏度下孵育24小时。菌株在有葡萄糖和无葡萄糖的情况下进行处理，分别在1、3和6分钟时分为对照（未处理），LED光（660 nm，166 mW / cm（2）），光敏剂（100μM亚甲蓝）和PACT。照射组。计数菌落形成单位，并将数据提交统计分析（ANOVA）和Tukey检验。结果：PACT能使白色念珠菌失活，并且葡萄糖的存在对大多数菌株的杀伤作用没有影响。仅YEM12受到其存在的部分影响。关于外排系统，ABC过表达菌株对酵母细胞显示出保护作用。我们观察到，主要促进子超家族（MFS）膜孔过表达的酵母倾向于在其细胞质中积累更多的MB，而过表达ABC泵（ATP结合盒转运蛋白）的菌株则倾向于减少MB的吸收并在光动力挑战中生存下来。葡萄糖的存在对PACT的影响很小。 MB在酵母上的积累会引起更多的光动力学失活；然而，光动力功效取决于微生物菌株的类型和特征。

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