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Evaluation of effect of two different functionalized nanoparticle photodynamic therapy on nanohardness of root dentin-An in vitro study

机译:两种不同官能化纳米粒子光动力治疗对根牙本肽纳米液体的影响的评价

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Introduction: The aim of this study was to evaluate the effect of functionalized nanoparticle photodynamic therapy on Nano hardness of root dentinMethodology: Fifty single rooted lower premolars were decoronated and sectioned into two halves. Then the samples were embedded horizontally in to the acrylic resin to expose the dentin surface. Baseline nanohardness was done at midroot level using a Nanohardness tester. Exposed dentin surfaces were immersed in the following irrigating solutionsGroup 1: 1 ml of Chitosan conjugated Rose Bengal (CSRB-np) activated with 540 nm for 10 minGroup 2: 1 ml of Methylene blue loaded poly lactic acid (PLGA-MB np) activated with 660 nm for 10 minGroup 3: 5 ml of 5.25% sodium hypochlorite for 1 min + 5 ml of 17% EDTA for 1 minGroup 4: 5 ml of 5.25% sodium hypochlorite for 1 min + 5 ml of 17% EDTA for 1 min + 1 ml of 2% chlorhexidine for 30 s.Group 5: 1 ml of 0.9 % Saline for 10 min.Post treatment nanohardness testing was done and results were analyzed statisticallyResults: In general, all the samples in their respective groups had significant change in nanohardness following immersion in irrigant solutions except in NaOCl + EDTA and saline group. CSRB-np and PLGA-MBnp showed increased nanohardness (P = 0.005 and P = 0.007 respectively). Whereas NaOCl + EDTA + CHX showed decrease in nanohardness (P = 0.04). With regards to Modulus of elasticity (MOE), CSRB-np showed significant difference (P = 0.002) compared to the other groups. MOE increased in CSRB-np and PLGA-MBnp while it decreased in all the other groups.Conclusion: In this study, the improvement of nanohardness and modulus of elasticity following the immersion of root dentin in CSRB-np solution was demonstrated
机译:介绍:本研究的目的是评估官能化纳米粒子光动力治疗对根牙本质学的纳米硬度的影响:用50个单根较低的磨牙,饰有两半。然后将样品水平嵌入丙烯酸树脂中以暴露牙本质表面。使用纳米腔体测试仪在Midroot水平下进行基线纳米性。将暴露的牙本质表面浸入下列灌溉溶液中的1:1ml壳聚糖缀合的玫瑰孟加拉(CSRB-NP),用540nm活化,10 mingroup 2:1ml活化的1ml亚甲基蓝载的聚乳酸(PLGA-MB NP) 10 mingroup 3:5ml 5.25%次氯酸钠1分钟+ 5ml 17%EDTA,1 mingroup 4:5ml 5.25%次氯酸钠1分钟+ 5ml 17%EDTA 1分钟+对于30秒的植物5:1ml的0.9%盐水10mL的1ml 2%盐酸盐10分钟。进行统计学上进行统计学评论结果,结果分析了统计学上的结果,其各自群体中的所有样品在纳急性中具有显着变化除了NaoCl + EDTA和盐碱外,浸入灌溉溶液中。 CSRB-NP和PLGA-MBNP显示出增加的纳米腔(P = 0.005和P = 0.007)。虽然NaoCl + EDTA + CHX显示纳米术(P = 0.04)下降。关于弹性模量(MOE),与其他基团相比,CSRB-NP显示出显着差异(p = 0.002)。 Moe在CSRB-NP和PLGA-MBNP中增加,而在所有其他基团中降低。结论:在本研究中,证明了CSRB-NP溶液中根牙本质浸没后的纳米型和弹性模量的改善

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