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Effects of violet radiation and nonthermal atmospheric plasma on the mineral contents of enamel during in-office dental bleaching

机译:紫色辐射和非热常压等离子体对办公牙科漂白期间牙釉质矿物质含量的影响

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Aim: This in vitro study assessed the effects of in-office bleaching with gels (35% hydrogen peroxide [HP] or 37% cabamide peroxide [CP]) and two activation sources (violet radiation [LED] or nonthermal atmospheric plasma [NTAP]) on the mineral content of bovine enamel.Material and methods: Dental blocks (n = 90) were assessed for initial microhardness before random distribution into nine groups: LED, LED + HP, LED + CP, NTAP, NTAP + HP, NTAP + CP, HP, CP and control (without treatment). Specimens were subjected to bleaching (2 clinical sessions, 7 days apart) using LED [20x/session, 1-min/each, 30s apart] or NTAP [1x/session, 10 min]. mu Raman determined contents of phosphate (PO43) and carbonate (CO32). Micro-energy dispersive X-ray fluorescence (mu EDXRF) and spectrophotometry of enamel microbiopsy evaluated the calcium to phosphorous ratios (Ca/P). Two-way ANOVA and Tukey tests analyzed mu RAMAN and mu EDXRF results. Spectrophotometry results were analyzed using Kruskal-Wallis and Dunn tests. Pearson correlation tested mu EDXRF and spectrophotometry results (alpha = 5%).Results: NTAP and NTAP + HP exhibited greater PO43- content than LED, LED + HP and control (p 0.05). No statistical differences were detected between CO32- among groups. While mu EDXRF evaluation demonstrated that NTAP and LED did not alter Ca/P ratio of enamel (p 0.05), spectrophotometry showed that Ca/P reduced for LED + HP (p 0.05). No correlation was found between mu EDXRF and enamel microbiopsy spectrophotometry (p 0.05).Conclusions: Activation sources did not adversely impact enamel's phosphate and carbonate concentrations after specimens' exposure to bleaching gels (either HP or CP). Visible light radiation emitted by a LED source was shown to adversely impact specimens' Ca/P ratios when treated with HP-containing bleaching gels.
机译:目的:这种体外研究评估了办公室漂白与凝胶的效果(35%过氧化氢[HP]或37%的Cabamide过氧化物[CP])和两个活化源(紫色辐射[LED]或非热大气血浆[NTAP]关于牛搪瓷的矿物质含量。材料和方法:在随机分布之前评估牙科嵌段(n = 90),以将初始显微性评估为九个组:LED,LED + HP,LED + CP,NTAP,NTAP + HP,NTAP + CP,HP,CP和控制(没有治疗)。使用LED [20x /次会议,1分钟/每种,30秒相距]或NTAP [1x /次,10分钟]对标本进行漂白(2个临床宿舍,7天)。 Mu拉曼确定磷酸盐(PO43)和碳酸酯(CO32)的含量。微能分散X射线荧光(MU EDXRF)和搪瓷微生物缺肌的分光光度法评估了磷比(CA / P)的钙。双向ANOVA和TUKEY测试分析了MU拉曼和MU EDXRF结果。使用Kruskal-Wallis和DUNN测试分析了分光光度法结果。 Pearson相关性测试MU EDXRF和分光光度法结果(alpha = 5%)。结果:NTAP和NTAP + HP表现出比LED,LED + HP和控制更大的PO43含量(P <0.05)。在CO 32之间没有检测到统计学差异。虽然MU EDXRF评估证明NTAP和LED未改变牙釉质的CA / P比(P> 0.05),分光光度法显示LED + HP的CA / P(P <0.05)。 Mu EDXRF和搪瓷微生物缺肌分光光度法没有发现相关性(P> 0.05)。结论:活化源在样品暴露于漂白凝胶(HP或CP)后不会产生不利影响搪瓷的磷酸盐和碳酸盐浓度。当用含HP的漂白凝胶处理时,由LED源发出的可见光辐射对样品的CA / P比率产生不利影响。

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