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Toward image quality assessment in optical coherence tomography (OCT) of rat kidney

机译:对大鼠肾脏光学相干断层扫描(OCT)的图像质量评估

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摘要

Background: Optical coherence tomography (OCT) is a useful tool for the evaluation of structure and function of the kidney, but the image quality can be effected by many factors. Objective: The objective of this study was to assess the image quality of different OCT systems in OCT imaging of the living kidney.Methods: One swept-source OCT (SSOCT) of 1300 nm, one spectral domain OCT (SDOCT) of 1300 nm and another of 900 nm were used. A FeO phantom was used to establish the point spread function (PSF). Rat kidneys were imaged for image quality assessment. Light penetration in the kidney and the optical attenuation coefficient were also evaluated. The quantification of uriniferous tubules was carried out via the threshold segmentation of 3D OCT images.Results: The quality of kidney images was resolution dependent. SDOCT of 900 nm showed higher peak signal-to noise ratio and dynamic range. The spatial resolution in the light field could be derived from the PSF distribution along three mutually orthogonal axes. In conjunction with the PSF, the Lucy-Richardson algorithm could improve image quality but could not reveal more microstructural information. The penetration depth of 1300 nm was deeper than that of 900 nm. The attenuation coefficient of the kidney was 29 cm (1) at 1300 nm and 50 cm(-1) at 900 nm (P 0.001). More accurate measurement of uriniferous tubules was achieved with the SDOCT-900 due to its higher resolution.Conclusions: Both SSOCT and SDOCT systems could be useful for imaging uriniferous tubules in the superficial layers of the cortex. The OCT image quality was highly correlated with the spatial resolution of OCT system.
机译:背景:光学相干断层扫描(OCT)是评估肾脏结构和功能的有用工具,但图像质量可以受到许多因素的影响。目的:本研究的目的是评估10月份肾脏成像的不同OCT系统的图像质量。方法:1300nm,一个光谱域10月(SDOCT)为1300nm和使用了900纳米的另一个。使用FEO幻影来建立点传播功能(PSF)。对图像质量评估进行了成像大鼠肾脏。还评估了肾脏和光学衰减系数的光渗透。通过3D OCT图像的阈值分割进行尿素管的定量。结果:肾脏图像的质量依赖于分辨率。 900nm的SDOCT显示出更高的峰值信号 - 信噪比和动态范围。光场中的空间分辨率可以沿着三个相互正交轴源自PSF分布。结合PSF,Lucy-Richardson算法可以提高图像质量,但无法透露更多的微观结构信息。 1300nm的渗透深度比900nm更深。肾脏的衰减系数在900nm的1300nm和50cm(-1)下为29cm(1)(p <0.001)。由于其较高的分辨率,通过SDOCT-900实现了更准确的尿素管测量。结论:SCOCT和SDOCT系统可用于在皮质的浅层层中成像尿素管。 OCT图像质量与OCT系统的空间分辨率高度相关。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2020年第12期|101983.1-101983.12|共12页
  • 作者单位

    Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Key Lab OptoElect Sci & Technol Med Fuzhou 350007 Peoples R China|Minnan Normal Univ Coll Phys & Informat Engn Zhangzhou 363000 Peoples R China;

    Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Key Lab OptoElect Sci & Technol Med Fuzhou 350007 Peoples R China;

    900th Hosp Joint Logist Support Force Dept Nephrol & Med Fuzhou 350000 Peoples R China;

    Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Key Lab OptoElect Sci & Technol Med Fuzhou 350007 Peoples R China;

    900th Hosp Joint Logist Support Force Dept Nephrol & Med Fuzhou 350000 Peoples R China;

    Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Key Lab OptoElect Sci & Technol Med Fuzhou 350007 Peoples R China;

    Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Key Lab OptoElect Sci & Technol Med Fuzhou 350007 Peoples R China|Univ Colorado Dept Elect Engn Denver CO 80202 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Optical coherence tomography; Kidney; Image quality; Point spread function (PSF); Quantification; Rat;

    机译:光学相干断层扫描;肾脏;图像质量;点传播功能(PSF);量化;老鼠;

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