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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Influences of galactose ligand on the uptake of TADF liposomes by HepG~2 cells
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Influences of galactose ligand on the uptake of TADF liposomes by HepG~2 cells

机译：半乳糖配体对Hepg〜2细胞吸收Tadf脂质体吸收的影响

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摘要

Glucose is the main energy substance to drive the physiological events of the cell.. Malignant cells exhibit a much higher rate of glycolysis than healthy cells to relieve the increased needs of energy. The higher metabolic rate induces the over-expression of the Glucose Transporter (GLUT) to transport more glucose into malignant cells. Our research regarded overexpressive GLUT as a target of nanoparticles. Substrate of GLUT galactose conjugated Polyethylene glycol-Distearyl phosphatidyl ethanolamine (PEG-DSPE) as a kind of ligand was selected to modified liposome. Thermally activated delayed fluorescence (TADF) was encapsulated as fluorescent probe to evaluate its abilities of targeting malignant cells, and the results of confocal microscopy and flow cytometry demonstrated that Galactose-PEG-DSPE modified liposome had the stronger efficiency of cellular uptake by HepG2 cells compared with Blank-PEG-DSPE modified liposome. The effect of GLUT1 inhibitor on cellular uptake of Galactose-PEG-DSPE modified liposomes showed that the mechanism might be relative to Warburg effect causing GLUT overexpression.
机译：葡萄糖是驱动细胞生理事件的主要能量物质。恶性细胞表现出比健康细胞更高的糖醇速率，以减轻能量的增加的需求。较高的代谢速率诱导葡萄糖转运蛋白（凝乳）的过表达，以将更多葡萄糖转化为恶性细胞。我们的研究将过度抑制呈纳米颗粒的靶标进行过度抑制。选择釉半乳糖缀合的聚乙二醇 - 二醇 - 二醇磷脂酰乙醇胺（PEG-DSPE）作为一种配体，以改性脂质体。将热活化的延迟荧光（TADF）包封成荧光探针，以评估靶向恶性细胞的能力，并且共聚焦显微镜和流式细胞术的结果表明，半乳糖-PEG-DSPE改性脂质体比较了HEPG2细胞的细胞吸收效率较强的效率用空白PEG-DSPE改性脂质体。 Glut1抑制剂对半乳糖-PEG-DSPE改性脂质体细胞摄取的影响表明，该机制可能是相对于导致肺过度表达的Warburg效应。

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  • 来源
    《Photodiagnosis and Photodynamic Therapy》 |2020年第12期|102014.1-102014.7|共7页
  • 作者

    Wang Chunyue; Chen Zhong; Tang Xuefeng; Liu Xiaoying; Wang Na; Li Wenhua; Liu Ting;

  • 作者单位

    Chinese Acad Med Sci & Peking Union Med Coll Natl Ctr Cardiovasc Dis State Key Lab Cardiovasc Dis Fuwai Hosp Beijing Peoples R China|Daqing Campus Harbin Med Univ 1 Xinyang Rd Daqing 163319 Peoples R China;

    Daqing Campus Harbin Med Univ 1 Xinyang Rd Daqing 163319 Peoples R China;

    Daqing Campus Harbin Med Univ 1 Xinyang Rd Daqing 163319 Peoples R China;

    Daqing Campus Harbin Med Univ 1 Xinyang Rd Daqing 163319 Peoples R China;

    Daqing Campus Harbin Med Univ 1 Xinyang Rd Daqing 163319 Peoples R China;

    Harbin Univ Commerce Sch Pharm 138 Tong Da St Harbin 150076 Peoples R China|Daqing Campus Harbin Med Univ 1 Xinyang Rd Daqing 163319 Peoples R China;

    Daqing Campus Harbin Med Univ 1 Xinyang Rd Daqing 163319 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Galactose; Liposome; GLUT; HepG2 cells; Warburg effect;

    机译：半乳糖;脂质体;露齿;hepg2细胞;Warburg效果;

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