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首页> 外文期刊>Physica Status Solidi. C, Conferences and critical reviews >CdS-Cd(OH)_2 core shell quantum dots functionalized with Concanavalin A lectin for recognition of mammary tumors
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CdS-Cd(OH)_2 core shell quantum dots functionalized with Concanavalin A lectin for recognition of mammary tumors

机译：用伴刀豆球蛋白A凝集素功能化的CdS-Cd（OH）_2核壳量子点识别乳腺肿瘤

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摘要

We report the use of CdS/Cd(OH)_2 quantum dots functionalized with glutaraldehyde and conjugated to concanavalin-A (Con-A) lectin to investigate cell alterations regarding carbohydraie profile in human mammary tissues diagnosed as fibroadenoma (benigne tumor). The Con-A lectin is a biomolecule which binds specifically to glucose/mannose residues present in the cellular membrane. These bioconjugated-particles were incubated with tissue sections of normal and to Fibroadenoma, a benign type of mammary tumor. The tissue sections were deparafinized, hydrated in graded alcohol and treated with a solution of Evans Blue in order to avoid autofluorescence. The fluorescence intensity of QD-Con-A stained tissues showed different patterns which reflect the carbohydrate expression of glucose/mannose in fibroadenoma when compared to the detection of the normal carbohydrate expression. The pattern of inespecific labeling of the tissues with glutharaldehyde functionalized CdS/Cd(OH)_2 quantum dots is compared to the targeting driven by the Con-A lectin. The preliminary findings reported here support the use of CdS/Cd(OH)_2 quantum dots as specific probes of cellular alterations possibiliting their use in diagnostics.
机译：我们报告了使用CdS / Cd（OH）_2量子点与戊二醛功能化并偶联到伴刀豆球蛋白A（Con-A）凝集素的用途，以研究关于被诊断为纤维腺瘤（良性肿瘤）的人类乳腺组织中碳水化合物分布的细胞变化。 Con-A凝集素是一种生物分子，可与细胞膜中存在的葡萄糖/甘露糖残基特异性结合。将这些生物共轭颗粒与正常组织和良性类型的乳腺纤维瘤（Fibroadenoma）的组织切片一起温育。将组织切片脱脂，在分级的乙醇中水合，并用伊文思蓝溶液处理以避免自发荧光。与检测正常碳水化合物表达相比，QD-Con-A染色的组织的荧光强度显示出不同的模式，反映了纤维腺瘤中葡萄糖/甘露糖的碳水化合物表达。将用戊二醛官能化的CdS / Cd（OH）_2量子点对组织进行非特异性标记的模式与由Con-A凝集素驱动的靶向进行比较。此处报道的初步发现支持将CdS / Cd（OH）_2量子点用作细胞改变的特定探针，从而可能将其用于诊断。

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