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首页> 外文会议>Colloidal Quantum Dots for Biomedical Applications; Progress in Biomedical Optics and Imaging; vol.7 no.19 >Lectin functionalized quantum dots for recognition of mammary tumors
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Lectin functionalized quantum dots for recognition of mammary tumors

机译：凝集素功能化量子点识别乳腺肿瘤

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摘要

In this study we use CdS/Cd(OH)_2 quantum dots functionalized with concanavalin-A (Con-A) lectin, specific to glucose/mannose residues, to investigate cell alterations regarding carbohydrate profile in human mammary tissues diagnosed as fibroadenoma (benign tumor). These particles were functionalized with glutaraldehyde and Con-A and incubated with tissue sections of normal and to Fibroadenoma, a benign type of mammary tumor. The tissue sections were deparafmized, hydrated in graded alcohol and treated with a solution of Evans Blue in order to avoid autofluorescence. The fluorescence intensity of QD-Con-A stained tissues showed different patterns, which reflect the carbohydrate expression of glucose/mannose in fibroadenoma when compared to the detection of the normal carbohydrate expression. The pattern of unspecific labeling of the tissues with glutaraldehyde functionalized CdS/Cd(OH)_2 quantum dots is compared to the targeting driven by the Con-A lectin. The preliminary findings reported here support the use of CdS/Cd(OH)_2 quantum dots as specific probes of cellular alterations and their use in diagnostics.
机译：在这项研究中，我们使用经伴刀豆球蛋白-A（Con-A）凝集素功能化的CdS / Cd（OH）_2量子点，特异于葡萄糖/甘露糖残基，来研究关于被诊断为纤维腺瘤（良性肿瘤）的人类乳腺组织中碳水化合物谱的细胞变化）。这些颗粒用戊二醛和Con-A功能化，并与正常组织和Fibroadenoma（一种良性乳腺肿瘤）的组织切片一起温育。将组织切片脱参数，在分级酒精中水合，并用伊文思蓝溶液处理以避免自发荧光。 QD-Con-A染色的组织的荧光强度显示出不同的模式，与检测正常碳水化合物表达相比，反映了纤维腺瘤中葡萄糖/甘露糖的碳水化合物表达。将戊二醛官能化的CdS / Cd（OH）_2量子点对组织的非特异性标记模式与由Con-A凝集素驱动的靶向进行比较。此处报道的初步发现支持将CdS / Cd（OH）_2量子点用作细胞改变的特定探针及其在诊断中的用途。

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