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首页> 外文期刊>Planetary and space science >Extraction of polar and nonpolar biomarkers from the martian soil using aqueous surfactant solutions
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Extraction of polar and nonpolar biomarkers from the martian soil using aqueous surfactant solutions

机译：使用表面活性剂水溶液从火星土壤中提取极性和非极性生物标志物

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摘要

The Life Marker Chip is being designed to detect the chemical evidence of life in the martian soil. It will use an aqueous surfactant solution to extract polar and nonpolar biomarkers from the martian soil and to transport them into an antibody-based detector for characterisation. Currently, a solution of 1.5 g I"1 polysorbate 80 in 20:80 (vohvol) methanol:water is being considered and appears to be suitable. Here, we have investigated the ability of a range of other surfactant solutions to extract a suite of eight standards spiked on the surfaces of the martian soil simulant JSC Mars-1 and tested the compatibility of the best two surfactants with a representative antibody assay for the detection of pyrene. The results show that using 20:80 (vol:vol) methanokwater as the solvent leads to increased recoveries of standards than using water alone. The poloxamer surfactants PluronicRE F-68 and PluronicRE F-108 are not effective at extracting the standards from JSC Mars-1 at any of the concentrations tested here. The fluorosurfactant ZonylRE FS-300 is able to extract the standards, but not as efficiently as polysorbate 80 solutions. Most successful of the alternative surfactants was the polysiloxane poly[dimethylsilox-ane-co-[3-(2-(2-hydroxyethoxy)ethoxy)propyl]methylsiloxane] (PDMSHEPMS) which is able to extract the standards from JSC Mars-1 with an efficiency approximately equal to that of polysorbate 80 solutions of the same concentration. Enhanced recovery of the standards using polysorbate 80 and PDMSHEPMS solutions can be achieved by increasing the concentration of surfactant, from 1.5 g I"1 to 10 gl~(-1), leading to an increase in the recovery of standards of about 50%. Polysorbate 80 at concentrations of 1.5 gl~(-1) and 10 gl~(-1) and ZonylRE FS-300 and PDMSHEPMS (both at a concentration of 10 gl~(-1) are also compatible with the representative pyrene antibody assay.
机译：生命标记芯片旨在检测火星土壤中生命的化学证据。它将使用表面活性剂水溶液从火星土壤中提取极性和非极性生物标志物，并将其运输到基于抗体的检测器中进行表征。目前，正在考虑将1.5 g I“ 1聚山梨酯80在20:80（体积）甲醇：水中的溶液考虑为合适的溶液。在这里，我们研究了多种其他表面活性剂溶液提取一套在火星土壤模拟物JSC Mars-1的表面上加标了8种标准品，并用代表性的抗体测定法检测了最好的两种表面活性剂与pyr的相容性，结果表明，使用20:80（vol：vol）的甲than水作为溶剂导致的标准品回收率比单独使用水增加。泊洛沙姆表面活性剂PluronicRE F-68和PluronicRE F-108不能有效地从JSC Mars-1中提取标准品，在任何测试浓度下都有效。 300能够提取标准液，但效率不如聚山梨酯80溶液，最成功的替代表面活性剂是聚硅氧烷聚[二甲基硅氧烷基-co- [3-（2-（2-羟基乙氧基）乙氧基）丙基]甲基[硅氧烷]（PDMSHEPMS）能够从JSC Mars-1提取标准品，其效率大约等于相同浓度的聚山梨酯80溶液的效率。使用聚山梨酯80和PDMSHEPMS溶液可提高标准品的回收率，方法是将表面活性剂的浓度从1.5 g I-1增至10 gl-1（-1），从而使标准品回收率提高约50％。浓度为1.5 gl〜（-1）和10 gl〜（-1）的聚山梨酯80以及ZonylRE FS-300和PDMSHEPMS（浓度均为10 gl〜（-1）也与代表性的sorb抗体测定兼容。

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  • 来源
    《Planetary and space science》 |2012年第1期|p.109-118|共10页
  • 作者

    Richard W. Court; Catherine S. Rix; Mark R. Sims; David C. Cullen; Mark A. Sephton;

  • 作者单位

    Department of Earth Science and Engineering, South Kensington Campus, Imperial College London SW7 2AZ, UK;

    Cranfield Health, Vincent Building, Cranfield University, Cranfield, Bedfordshire MK43 OAL, UK;

    Space Research Centre, Michael Atiyah Building Department of Physics and Astronomy, University of Leicester, Leicester LE17RH, UK;

    Cranfield Health, Vincent Building, Cranfield University, Cranfield, Bedfordshire MK43 OAL, UK;

    Department of Earth Science and Engineering, South Kensington Campus, Imperial College London SW7 2AZ, UK;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    mars; exomars; extraterrestrial life;

    机译：火星;exomas;地球外的生活;

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