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首页> 外文期刊>Plant Cell Reports >Development of a transgenic hairy root system in jute (Corchorus capsularis L.) with gusA reporter gene through Agrobacterium rhizogenes mediated co-transformation
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Development of a transgenic hairy root system in jute (Corchorus capsularis L.) with gusA reporter gene through Agrobacterium rhizogenes mediated co-transformation

机译：通过发根农杆菌介导的共转化，开发具有gusA报告基因的黄麻（Corchorus荚ris）转基因毛状根系统

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摘要

Transgenic hairy root system is important in several recalcitrant plants, where Agrobacterium tumefaciens-mediated plant transformation and generation of transgenic plants are problematic. Jute (Corchorus spp.), the major fibre crop in Indian subcontinent, is one of those recalcitrant plants where in vitro tissue culture has provided a little success, and hence, Agrobacterium-mediated genetic transformation remains to be a challenging proposition in this crop. In the present work, a system of transgenic hairy roots in Corchorus capsularis L. has been developed through genetic transformation by Agrobacterium rhizogenes harbouring two plasmids, i.e. the natural Ri plasmid and a recombinant binary vector derived from the disarmed Ti plasmid of A. tumefaciens. Our findings indicate that the system is relatively easy to establish and reproducible. Molecular analysis of the independent lines of transgenic hairy roots revealed the transfer of relevant transgenes from both the T-DNA parts into the plant genome, indicating the co-transformation nature of the event. High level expression and activity of the gusA reporter gene advocate that the transgenic hairy root system, thus developed, could be applicable as gene expression system in general and for root functional genomics in particular. Furthermore, these transgenic hairy roots can be used in future as explants for plantlet regeneration to obtain stable transgenic jute plants.
机译：转基因的毛状根系统在一些顽固的植物中很重要，在这些植物中，根癌农杆菌介导的植物转化和转基因植物的产生是有问题的。黄麻（Corchorus spp。）是印度次大陆的主要纤维作物，是那些顽tissue的植物之一，其体外组织培养取得了一些成功，因此，农杆菌介导的遗传转化在该作物中仍然是具有挑战性的命题。在目前的工作中，通过发根农杆菌通过遗传转化已经开发了荚膜Corchorus荚膜L.中的转基因毛状根的系统，该农杆菌携带两种质粒，即天然Ri质粒和衍生自根癌农杆菌的解除武装的Ti质粒的重组二元载体。我们的发现表明，该系统相对易于建立和重现。对转基因毛根独立株系的分子分析表明，相关的转基因从两个T-DNA部分转移到了植物基因组中，表明了该事件的共转化性质。 gusA报告基因的高水平表达和活性表明，这样开发的转基因毛状根系统可以普遍用作基因表达系统，尤其适用于根功能基因组学。此外，这些转基因有毛的根将来可以用作用于苗再生的外植体以获得稳定的转基因黄麻植物。

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