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首页> 外文期刊>Plant Cell Reports >Three cotton homeobox genes are preferentially expressed during early seedling development and in response to phytohormone signaling
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Three cotton homeobox genes are preferentially expressed during early seedling development and in response to phytohormone signaling

机译：三种棉花同源异型盒基因在幼苗早期发育期间以及对植物激素信号的响应中优先表达

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摘要

Homeodomain-leucine zipper (HD-Zip) proteins are transcription factors unique to plants. In this study, three cDNAs (designated as GhHB2, GhHB3 and GhHB4) encoding HD-Zip proteins were isolated from cotton cDNA library. GhHB2 gene encodes a protein of 300 amino acids, GhHB3 gene encodes a peptide with 254 amino acids, and GhHB4 gene encodes a protein of 281 amino acids. The deduced proteins, which contain the homeodomain and leucine-rich zipper motif, share relatively high similarities with the other plant HD-Zip proteins. Quantitative RT-PCR analysis indicated that GhHB3 and GhHB4 were preferentially expressed in hypocotyls and cotyledons, whereas GhHB2 gene was predominantly expressed in young stems, at relatively high levels in hypocotyls. Expressions of all the three genes were up-regulated in roots, hypocotyls and cotyledons after GA3 treatments. Additionally, GhHB4 expression was enhanced by 6-BA treatment. A GhHB2 promoter fragment was isolated from cotton by Genome-Walking PCR. Expression of GUS gene controlled under GhHB2 promoter was examined in the transgenic Arabidopsis plants. Strong GUS staining was detected in cotyledon, veins of the emerging leaves and shoot apices of 5- to 15-day-old transgenic seedlings, but GUS activity became more and more weak as the seedlings further developed. In addition, the promoter activity was induced by exogenous GA, indicating that GhHB2 promoter is very active during early seedling development, and may be GA-inducible. The results suggested that the three HB genes may function in early seedling development of cotton and in response to gibberellin signaling.
机译：同源异域亮氨酸拉链（HD-Zip）蛋白是植物特有的转录因子。在这项研究中，从棉花cDNA文库中分离了三个编码HD-Zip蛋白的cDNA（分别称为GhHB2，GhHB3和GhHB4）。 GhHB2基因编码300个氨基酸的蛋白质，GhHB3基因编码254个氨基酸的肽，GhHB4基因编码281个氨基酸的蛋白质。推导的蛋白质包含同源结构域和富含亮氨酸的拉链基序，与其他植物HD-Zip蛋白质具有相对较高的相似性。定量RT-PCR分析表明，GhHB3和GhHB4在下胚轴和子叶中优先表达，而GhHB2基因主要在幼茎中表达，在下胚轴中相对较高。 GA 3 处理后，这三个基因在根，下胚轴和子叶中的表达均上调。另外，通过6-BA处理增强了GhHB4表达。通过基因组步行PCR从棉花中分离出GhHB2启动子片段。在转基因拟南芥植物中检查了受GhHB2启动子控制的GUS基因的表达。在5到15天大的转基因幼苗的子叶，叶片的叶脉和芽尖中检测到强GUS染色，但是随着幼苗的进一步发育，GUS活性变得越来越弱。另外，启动子活性是由外源GA诱导的，表明GhHB2启动子在幼苗早期发育期间非常活跃，并且可能是GA诱导的。结果表明，这三个HB基因可能在棉花的幼苗生长早期以及对赤霉素信号的响应中起作用。

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