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首页> 外文期刊>Plant Cell Reports >Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars
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Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars

机译：茄子林之间体细胞杂种的多重抗性特性的表征。和两个商业马铃薯品种

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Interspecific somatic hybrids between commercial cultivars of potato Solanum tuberosum L. Agave and Delikat and the wild diploid species Solanum cardiophyllum Lindl. (cph) were produced by protoplast electrofusion. The hybrid nature of the regenerated plants was confirmed by flow cytometry, simple sequence repeat (SSR), amplified fragment length polymorphism (AFLP), microsatellite-anchored fragment length polymorphism (MFLP) markers and morphological analysis. Somatic hybrids were assessed for their resistance to Colorado potato beetle (CPB) using a laboratory bioassay, to Potato virus Y (PVY) by mechanical inoculation and field trials, and foliage blight in a greenhouse and by field trials. Twenty-four and 26 somatic hybrids of cph + cv. Agave or cph + cv. Delikat, respectively, showed no symptoms of infection with PVY, of which 3 and 12, respectively, were also resistant to foliage blight. One hybrid of cph + Agave performed best in CPB and PVY resistance tests. Of the somatic hybrids that were evaluated for their morphology and tuber yield in the field for 3 years, four did not differ significantly in tuber yield from the parental and standard cultivars. Progeny of hybrids was obtained by pollinating them with pollen from a cultivar, selfing or cross-pollination. The results confirm that protoplast electrofusion can be used to transfer the CPB, PVY and late blight resistance of cph into somatic hybrids. These resistant somatic hybrids can be used in pre-breeding studies, molecular characterization and for increasing the genetic diversity available for potato breeding by marker-assisted combinatorial introgression into the potato gene pool.
机译：马铃薯Solaum tuberosum L. Agave和Delikat的商业栽培品种与野生二倍体物种Solanum cardiophyllum Lindl之间的种间体细胞杂种。 （cph）是通过原生质体电融合产生的。通过流式细胞术，简单序列重复（SSR），扩增片段长度多态性（AFLP），微卫星锚定片段长度多态性（MFLP）标记和形态分析证实了再生植物的杂种性质。使用实验室生物测定法评估体细胞杂种对科罗拉多马铃薯甲虫（CPB）的抗性，通过机械接种和田间试验以及温室中的叶枯病和田间试验对马铃薯病毒Y（PVY）的抗性。 cph + cv的二十四个和26个体细胞杂种。龙舌兰或cph + cv。 Delikat分别没有显示出PVY感染的症状，其中分别有3和12个对叶枯病也有抵抗力。 cph +龙舌兰的一种杂种在CPB和PVY抗性测试中表现最好。在三年中对其形态和块茎产量进行评估的体细胞杂种中，有四个与亲本和标准品种的块茎产量无明显差异。杂种的后代是通过用自交或异花授粉的花粉对它们进行授粉而获得的。结果证实，原生质体电融合可用于将CPH的CPB，PVY和晚疫病抗性转移到体细胞杂种中。这些抗性体细胞杂种可用于预育研究，分子鉴定和通过标记辅助组合渗入马铃薯基因库来增加马铃薯育种可用的遗传多样性。

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