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首页> 外文期刊>Plant Cell Reports >Expressions of three cotton genes encoding the PIP proteins are regulated in root development and in response to stresses
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Expressions of three cotton genes encoding the PIP proteins are regulated in root development and in response to stresses

机译：三种棉花PIP蛋白基因的表达在根系发育和逆境胁迫中受到调节

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Cotton (Gossypium hirsutum), the most important textile crop worldwide, often encounters water stress such as drought or waterlog during its growth season (Summer). To investigate molecular mechanism of water regulation in cotton plants, three cDNAs encoding the plasma membrane intrinsic protein (PIP) were isolated from cotton root cDNA library, and designated GhPIP1;1, GhPIP2;1 and GhPIP2;2, respectively. All of the three PIP proteins displayed water channel activity in Xenopus laevis oocytes. GhPIP2;1 and GhPIP2;2 proteins, however, showed much higher water transport activity than that of the GhPIP1;1 protein. Northern blot analysis revealed that all of the three genes were preferentially expressed in young roots. Further analysis by Real-time quantitative RT-PCR revealed that the transcripts of all the three genes were accumulated at high levels in 3-day-old young roots, but dramatically declined to much lower levels in 6–14 days old roots during seedling development, suggesting that expressions of the isolated GhPIP genes are developmentally regulated in roots. Additionally, expressions of the three genes were remarkably up-regulated or down-regulated under different stresses such as NaCl, cold, PEG (polyethylene glycol) treatments. Collectively, the results suggest that these genes may be involved in root development and in response to stresses.
机译：棉花（陆地棉）是世界上最重要的纺织作物，在其生长季节（夏季）经常遇到水分胁迫，例如干旱或内涝。为了研究棉花植物水分调节的分子机制，从棉花根cDNA文库中分离出三个编码质膜内在蛋白（PIP）的cDNA，分别命名为GhPIP1; 1，GhPIP2; 1和GhPIP2; 2。这三种PI​​P蛋白均在非洲爪蟾卵母细胞中显示出水通道活性。但是，GhPIP2; 1和GhPIP2; 2蛋白显示的水转运活性比GhPIP1; 1蛋白高。 Northern印迹分析显示，这三个基因全部在幼根中优先表达。通过实时定量RT-PCR的进一步分析显示，所有三个基因的转录本均在3天大的幼根中高水平积累，但在幼苗发育过程中，在6-14天大的根中急剧下降至低得多的水平，表明分离的GhPIP基因的表达在根中受到发育调控。另外，在不同的胁迫下，例如NaCl，冷，PEG（聚乙二醇）处理，这三个基因的表达显着上调或下调。总的来说，这些结果表明这些基因可能参与了根的发育和对压力的反应。

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