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首页> 外文期刊>Plant Cell Reports >Pistil-function breakdown in a new S-allele of European pear, S21°, confers self-compatibility
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Pistil-function breakdown in a new S-allele of European pear, S21°, confers self-compatibility

机译：欧洲梨的一个新的S-等位基因S 21 °的雌激素功能分解赋予了自相容性

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European pear exhibits RNase-based gametophytic self-incompatibility controlled by the polymorphic S-locus. S-allele diversity of cultivars has been extensively investigated; however, no mutant alleles conferring self-compatibility have been reported. In this study, two European pear cultivars, ‘Abugo’ and ‘Ceremeño’, were classified as self-compatible after fruit/seed setting and pollen tube growth examination. S-genotyping through S-PCR and sequencing identified a new S-RNase allele in the two cultivars, with identical deduced amino acid sequence as S 21 , but differing at the nucleotide level. Test-pollinations and analysis of descendants suggested that the new allele is a self-compatible pistil-mutated variant of S 21 , so it was named S 21 °. S-genotypes assigned to ‘Abugo’ and ‘Ceremeño’ were S 10 S 21 ° and S 21 °S 25 respectively, of which S 25 is a new functional S-allele of European pear. Reciprocal crosses between cultivars bearing S 21 and S 21 ° indicated that both alleles exhibit the same pollen function; however, cultivars bearing S 21 ° had impaired pistil-S function as they failed to reject either S 21 or S 21 ° pollen. RT-PCR analysis showed absence of S 21 °-RNase gene expression in styles of ‘Abugo’ and ‘Ceremeño’, suggesting a possible origin for S 21 ° pistil dysfunction. Two polymorphisms found within the S-RNase genomic region (a retrotransposon insertion within the intron of S 21 ° and indels at the 3′UTR) might explain the different pattern of expression between S 21 and S 21 °. Evaluation of cultivars with unknown S-genotype identified another cultivar ‘Azucar Verde’ bearing S 21 °, and pollen tube growth examination confirmed self-compatibility for this cultivar as well. This is the first report of a mutated S-allele conferring self-compatibility in European pear.
机译：欧洲梨表现出由多态性S基因座控制的基于RNase的配子体自我不相容性。品种的S-等位基因多样性已得到广泛研究。然而，尚未报道赋予自我相容性的突变等位基因。在这项研究中，两个欧洲梨品种“ Abugo”和“Ceremeño”经过水果/种子镶嵌和花粉管生长检查后被归类为自相容。通过S-PCR和测序进行S基因分型，在两个品种中鉴定出一个新的S-RNase等位基因，其推导的氨基酸序列与S 21 相同，但在核苷酸水平上有所不同。传粉和后代的分析表明，新的等位基因是S 21 的自相容雌蕊突变变体，因此被命名为S 21 °。分配给“ Abugo”和“Ceremeño”的S基因型是S 10 S 21 °和S 21 °S 25 ，其中S 25 是欧洲梨的新功能S等位基因。带有S 21 和S 21 °的品种之间的相互杂交表明，两个等位基因都表现出相同的花粉功能。然而，带有S 21 °的品种的雌蕊S功能受损，因为它们不能拒绝S 21 或S 21 °花粉。 RT-PCR分析显示，S 21 °-RNase基因不存在“ Abugo”和“Ceremeño”形式的表达，提示S 21 °雌蕊功能障碍的可能来源。在S-RNase基因组区域内发现的两个多态性（S 21 °内含子内的反转录转座子插入和3'UTR上的插入缺失）可能解释了S 21 < / sub>和S 21 °。对S基因型未知的品种的评估确定了另一个带有S 21 °的品种'Azucar Verde'，并且花粉管生长检查也证实了该品种的自我适应性。这是赋予欧洲梨自交性的突变S-等位基因的首次报道。

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    《Plant Cell Reports》 |2009年第3期|p.457-467|共11页
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    Javier Sanzol;

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