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首页> 外文期刊>Plant Cell Reports >Analysis of gene expression profile in pollen development of recessive genic male sterile Brassica napus L. line S45A
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Analysis of gene expression profile in pollen development of recessive genic male sterile Brassica napus L. line S45A

机译：隐性基因雄性不育甘蓝型油菜S45A花粉发育中的基因表达谱分析

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摘要

Male sterility in a near-isogenic line S45AB after 25 generations of subcrossing is controlled by two pairs of duplicate genes. The genotype of S45A is Bnms1Bnms1Bnms2Bnms2, and that of S45B is BnMs1Bnms1Bnms2Bnms2, respectively. Histological observations revealed that abnormal anther development appeared in the tapetum and pollen exine during the tetrad stage. This male sterility was characterized by hypertrophy of the tapetal cells at the tetrad stage and a complete lack of microspore exine after the release of microspores from the tetrads. To elucidate the mechanism of this recessive genic male sterility, the flower bud expression profiles of the S45A and S45B lines were analyzed using an Arabidopsis thaliana ATH1 oligonucleotide array. When compared with the S45B line, 69 genes were significantly downregulated, and 46 genes were significantly upregulated in the S45A line. Real-time polymerase chain reaction (PCR) was then used to verify the results of the microarray analysis, and the majority of the downregulated genes in the S45A line were abundantly and specifically expressed in the anther. The results of the real-time PCR suggest that Bnms1 might be involved in the metabolism of lipid/fatty acids, and the homologous mutation of Bnms1 may either block the biosynthesis of sporopollenin or block sporopollenin from being deposited on the microspore surface, thus, preventing pollen exine formation. The role of Bnms1 in the regulatory network of exine formation is also discussed as well.
机译：25代亚杂交后，近等基因系S45AB中的雄性不育由两对重复基因控制。 S45A的基因型分别为Bnms1Bnms1Bnms2Bnms2，S45B的基因型分别为BnMs1Bnms1Bnms2Bnms2。组织学观察表明，在四分体阶段，绒毡层和花粉外壁中出现了异常的花药发育。这种雄性不育的特征是在四分体阶段的绒毛膜细胞肥大，并且从四分体释放小孢子后完全缺乏小孢子外壁。为了阐明这种隐性遗传雄性不育的机理，使用拟南芥ATH1寡核苷酸阵列分析了S45A和S45B系的花芽表达谱。与S45B品系相比，S45A品系中有69个基因被显着下调，而46个基因被显着上调。然后使用实时聚合酶链反应（PCR）验证了微阵列分析的结果，S45A系中的大多数下调基因在花药中都大量表达并特异性表达。实时PCR的结果表明Bnms1可能参与脂质/脂肪酸的代谢，并且Bnms1的同源突变可能会阻止孢粉的生物合成或阻止孢粉的沉积在小孢子表面，从而防止花粉外生形成。还讨论了Bnms1在外膜形成的调控网络中的作用。

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