Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices

Nina Papazova; Rim Ghedira; Sabine Van Glabeke; Aghleb Bartegi; Pieter Windels; Isabel Taverniers; Isabel Roldan-Ruiz; Erik Van Bockstaele; Anne Milcamps; Guy Van Den Eede; Ann Depicker; Marc De Loose

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Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices

机译：非生物胁迫和栽培方式影响下拟南芥转基因植物T-DNA侧翼区的稳定性

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Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of the transgene flanking regions in the model plant Arabidopsis thaliana. Conformational Sensitive Capillary Electrophoresis (CSCE), RFLP and sequencing were deployed in this analysis in order to study the proximal 100 bp and the long-range T-DNA flanking sequences. By screening seven transgenic lines no evidence for occurrence of mutation events were found, implying that the nucleotide sequence of the T-DNA flanking regions of the studied events is unlikely to be unstable.

机译：遗传转化通常与植物基因组在插入位点的不同重排有关。因此，问题仍然在于这些T-DNA插入位点更容易遭受基因毒性胁迫。在这里，我们研究了模式植物拟南芥中世代繁殖，高光强度引起的基因堆叠和光氧化胁迫对转基因侧翼区稳定性的影响。为了研究近端100 bp和长距离T-DNA侧翼序列，在此分析中使用了构象敏感性毛细管电泳（CSCE），RFLP和测序。通过筛选七个转基因品系，没有发现突变事件发生的证据，这表明所研究事件的T-DNA侧翼区域的核苷酸序列不太可能是不稳定的。

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来源
《Plant Cell Reports》 |2008年第4期|749-757|共9页
作者
Nina Papazova; Rim Ghedira; Sabine Van Glabeke; Aghleb Bartegi; Pieter Windels; Isabel Taverniers; Isabel Roldan-Ruiz; Erik Van Bockstaele; Anne Milcamps; Guy Van Den Eede; Ann Depicker; Marc De Loose;
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Unit Technology and Food Institute for Agricultural and Fisheries Research Burg. Van Gansberghelaan 115 9820 Merelbeke Belgium;

Unit Technology and Food Institute for Agricultural and Fisheries Research Burg. Van Gansberghelaan 115 9820 Merelbeke Belgium;

Unit Plant Institute for Agricultural and Fisheries Research Caritasstraat 21 9090 Melle Belgium;

Unité de Recherche de Biochimie Institut Supérieur de Biotechnologie Rue Tahar Haddad 5000 Monastir Tunisia;

Unit Technology and Food Institute for Agricultural and Fisheries Research Burg. Van Gansberghelaan 115 9820 Merelbeke Belgium;

Unit Technology and Food Institute for Agricultural and Fisheries Research Burg. Van Gansberghelaan 115 9820 Merelbeke Belgium;

Unit Plant Institute for Agricultural and Fisheries Research Caritasstraat 21 9090 Melle Belgium;

Unit Plant Institute for Agricultural and Fisheries Research Caritasstraat 21 9090 Melle Belgium;

European Commission - Joint Research Centre Institute for Health and Consumer Protection Via E. Fermi 1 21020 Ispra (VA) Italy;

European Commission - Joint Research Centre Institute for Health and Consumer Protection Via E. Fermi 1 21020 Ispra (VA) Italy;

Department of Plant Systems Biology VIB Technologiepark 927 9052 Gent Belgium;

Unit Technology and Food Institute for Agricultural and Fisheries Research Burg. Van Gansberghelaan 115 9820 Merelbeke Belgium;

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正文语种 eng
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关键词
T-DNA flanking regions; Single nucleotide polymorphisms (SNPs); T-DNA insertion; GMO; Arabidopsis thaliana;

机译：T-DNA侧翼区;单核苷酸多态性（SNPs）;T-DNA插入;GMO;拟南芥;

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专利

1. Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices [J] . Papazova N, Ghedira R, Van Glabeke S, Plant Cell Reports . 2008,第4期

机译：非生物胁迫和栽培措施影响下拟南芥转基因植物T-DNA侧翼区的稳定性
2. Isolation of T-DNA flanking plant DNA from T-DNA insertional embryo-lethal mutants of Arabidopsis thaliana by plasmid rescue technique [J] . YAO XIAO LI, JIAN GE SUN, ZHI PING ZHU Cell research. . 1996,第2期

机译：利用质粒拯救技术从拟南芥T-DNA插入胚致死突变体中分离T-DNA侧翼植物DNA
3. Putrescine accumulation in Arabidopsis thaliana transgenic lines enhances tolerance to dehydration and freezing stress. (Special Issue: Plant abiotic stress.) [J] . Alet A. I., Sanchez D. H., Cuevas J. C., Plant signaling & behavior . 2011,第2期

机译：拟南芥转基因品系中的腐胺积累增强了对脱水和冰冻胁迫的耐受性。（特刊：植物非生物胁迫。）
4. Analysis of flanking sequences of T-DNAs in transgenic birch plants Based on SiteFinding PCR [C] . Fan-Suo ZENG, Ya-Guang ZHAN, Tao SU, The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议）论文集 . 2008

机译：基于SiteFinding PCR的转基因桦树植物T-DNA侧翼序列分析
5. Using T-DNA tagging to isolate novel genes from Arabidopsis essential for abiotic stress tolerance. [D] . Zhu, Jianhua. 2004

机译：使用T-DNA标签从拟南芥中分离出非生物胁迫耐受性必需的新基因。
6. An Intergenic Region Shared by At4g35985 and At4g35987 in Arabidopsis thaliana Is a Tissue Specific and Stress Inducible Bidirectional Promoter Analyzed in Transgenic Arabidopsis and Tobacco Plants [O] . Joydeep Banerjee, Dipak Kumar Sahoo, Nrisingha Dey, -1

机译：At4g35985和At4g35987在拟南芥中共有的一个基因间区域是组织特异性和胁迫诱导的双向启动子已在转基因拟南芥和烟草植物中进行了分析。
7. An intergenic region shared by At4g35985 and At4g35987 in Arabidopsis thaliana is a tissue specific and stress inducible bidirectional promoter analyzed in transgenic arabidopsis and tobacco plants. [O] . Joydeep Banerjee, Dipak Kumar Sahoo, Nrisingha Dey, 2013

机译：拟南芥中at4g35985和at4g35987共有的基因间区域是在转基因拟南芥和烟草植物中分析的组织特异性和胁迫诱导型双向启动子。

Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices

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