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首页> 外文期刊>Plant Cell Reports >Identification of differentially expressed transcripts from leaves of the boron tolerant plant Gypsophila perfoliata L.
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Identification of differentially expressed transcripts from leaves of the boron tolerant plant Gypsophila perfoliata L.

机译：从耐硼植物满天星满天星叶中鉴定差异表达的转录本。

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Very recently some of the species of Gypsophila genus collected from the boron rich soils in Turkey were shown to be remarkably tolerant to high levels of boron. A limited amount of boron is necessary for the normal development of plants; however, a high level of boron in soil is generally toxic. Nevertheless, the adaptability of plant species allows them to withstand the presence of extreme amounts of metal ion by various strategies. This study is conducted on highly boron tolerant Gypsophila perfoliata L. collected from a location in the boron mining area. The plant samples were transferred into plant nutritional medium in the presence high; ~500 (35 mg/kg), 1,000, and 30 μM (considered normal) boron concentrations. We compared the transcriptome of the plant sample treated with the excess levels of boron to that of the samples grown under normal concentration using differential display PCR (DDRT-PCR) method. Thirty bands showing differential expression levels (presence or absence of bands or varying intensities) in either of ~500 or 30 μM B concentrations at varying time points were excised, cloned, and sequenced. Among which, 18 of them were confirmed via quantitative reverse transcription real time PCR (qRT-PCR). We are reporting the first preliminary molecular level study of boron tolerance on this organism by attempting to identify putative genes related in the tolerance mechanism. The gene fragments are consistent with the literature data obtained from a proteomics study and a metabolomics study performed in barley under varying boron concentrations.
机译：最近，从土耳其富含硼的土壤中收集到的满天星属的某些物种显示出对高硼含量的耐受性。正常植物的生长需要少量的硼；但是，土壤中高含量的硼通常是有毒的。然而，植物物种的适应性使它们能够通过各种策略承受极端数量的金属离子的存在。这项研究是从硼矿区的一个地方收集的，对硼具有高度耐受性的满天星满天星进行的研究。将植物样品在高存在下转移到植物营养培养基中。约500（35 mg / kg），1,000和30μM（考虑为正常）的硼浓度。我们将用过量硼处理过的植物样品的转录组与使用差异显示PCR（DDRT-PCR）方法在正常浓度下生长的样品的转录组进行了比较。切除，克隆并测序了30条在不同时间点的〜500或30μMB浓度下显示差异表达水平的条带（条带的存在与否或强度变化）。其中，通过定量逆转录实时定量PCR（qRT-PCR）确认了其中的18种。我们正在尝试通过鉴定与耐受机制相关的推定基因，首次报道了对这种生物的硼耐受性的初步分子水平研究。这些基因片段与从蛋白质组学研究和在不同硼浓度下在大麦中进行的代谢组学研究获得的文献数据一致。

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