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首页> 外文期刊>Plant Cell Reports >Over-expression of tobacco NtHSP70-1 contributes to drought-stress tolerance in plants
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Over-expression of tobacco NtHSP70-1 contributes to drought-stress tolerance in plants

机译：烟草NtHSP70-1的过表达促进植物的干旱胁迫耐受性

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摘要

HSP70, a heat shock protein, is a molecular chaperone responsive to various environmental stresses. Here, NtHSP70-1 (AY372069) was a drought-/ABA-inducible gene. We monitored the expression of CaERD15 (early responsive to dehydration, DQ267932) with exposing plants to progressive drought stress. Its activity was used as an indicator of water-deficit conditions. To analyze the protective role of HSP70, we obtained transgenic tobacco plants that constitutively expressed elevated levels of the tobacco HSP70, NtHSP70-1, as well as transgenic plants containing either the vector alone or else having NtHSP70-1 in the antisense orientation. Plants with enhanced levels of NtHSP70-1 in their transgenic sense lines exhibited tolerance to water stress. Under progressive drought, the amount of leaf NtHSP70-1 was correlated with maintenance of optimum water content, with contents being higher in the leaves of dehydrated transgenic sense plants than in those of either the control (vector-only) or the transgenic antisense plants. Moreover, the expression of CaERD15 was considerably reduced in tobacco plants that over-expressed NtHSP70-1. These results suggest that elevated levels of NtHSP70-1 can confer drought-stress tolerance.
机译：HSP70是一种热休克蛋白，是对各种环境胁迫有反应的分子伴侣。在此，NtHSP70-1（AY372069）是干旱/ ABA诱导型基因。我们通过使植物暴露于渐进性干旱胁迫下来监测CaERD15的表达（对脱水早期反应，DQ267932）。它的活性被用作缺水状况的指标。为了分析HSP70的保护作用，我们获得了组成型表达烟草HSP70，NtHSP70-1水平升高的转基因烟草植物，以及仅包含载体或反义方向具有NtHSP70-1的转基因植物。在其转基因有义系中具有增强水平的NtHSP70-1的植物表现出对水分胁迫的耐受性。在渐进干旱下，叶片NtHSP70-1的量与维持最佳水分有关，脱水的转基因有义植物叶片中的含量高于对照（仅载体）或转基因反义植物中的含量。此外，在过表达NtHSP70-1的烟草植物中，CaERD15的表达大大降低。这些结果表明升高水平的NtHSP70-1可以赋予干旱胁迫耐受性。

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  • 来源
    《Plant Cell Reports》 |2006年第4期|349-358|共10页
  • 作者

    Eun Kyung Cho; Choo Bong Hong;

  • 作者单位

    Institute of Molecular Biology and Genetics and School of Biological Sciences Seoul National University Seoul 151-742 Korea;

    Institute of Molecular Biology and Genetics and School of Biological Sciences Seoul National University Seoul 151-742 Korea;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    CaERD15; Drought; NtHSP70-1; Tobacco; Transgenic plants; ABA;

    机译：CaERD15;干旱;NtHSP70-1;烟草;转基因植物;ABA;

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