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首页> 外文期刊>Plant Methods >Diel leaf growth of soybean: a novel method to analyze two-dimensional leaf expansion in high temporal resolution based on a marker tracking approach (Martrack Leaf)
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Diel leaf growth of soybean: a novel method to analyze two-dimensional leaf expansion in high temporal resolution based on a marker tracking approach (Martrack Leaf)

机译：大豆的Diel叶片生长：一种基于标记跟踪方法的高解析度二维叶片膨胀分析新方法（Martrack Leaf）

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摘要

Background We present a novel method for quantitative analysis of dicot leaf expansion at high temporal resolution. Image sequences of growing leaves were assessed using a marker tracking algorithm. An important feature of the method is the attachment of dark beads that serve as artificial landmarks to the leaf margin. The beads are mechanically constricted to the focal plane of a camera. Leaf expansion is approximated by the increase in area of the polygon defined by the centers of mass of the beads surrounding the leaf. Fluctuating illumination conditions often pose serious problems for tracking natural structures of a leaf; this problem is circumvented here by the use of the beads.
机译：背景技术我们提出了一种在高时间分辨率下对双子叶植物叶片扩展进行定量分析的新方法。使用标记跟踪算法评估生长叶片的图像序列。该方法的一个重要特征是将作为人造地标的深色珠子附着在叶缘上。磁珠机械收缩到相机的焦平面。叶片的膨胀可以通过多边形的面积增加来近似，该面积由围绕叶片的小珠的质心确定。光照条件的变化通常会给跟踪叶片的自然结构带来严重的问题。通过使用珠子可以解决这个问题。

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