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首页> 外文期刊>Polar biology >Characterization and comparison of potential denitrifiers in microbial mats from King George Island, Maritime Antarctica
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Characterization and comparison of potential denitrifiers in microbial mats from King George Island, Maritime Antarctica

机译：南极海洋国王乔治岛微生物垫中潜在反硝化剂的表征和比较

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摘要

Cyanobacterial microbial mats are highly structured communities commonly found in Antarctic inland waters including melt streams. These benthic microbial associations comprise a large number of microorganisms with different metabolic capacities, impacting nutrient dynamics where established. The denitrification process is a feasible nitrogen loss pathway and a biological source of nitrous oxide, a potent greenhouse gas that also promotes ozone depletion. Potential denitrifiers from five microbial mats were characterized using a PCR-DGGE (denaturing gradient gel electro-phoresis) approach. Molecular markers encoding for key enzymes in the denitrification process (nirK, nirS and nosZ) were used. Fingerprints were obtained for the five sampled mats and compared for two successive years. Distance analysis showed that despite the sampled year, the denitrifying genetic potential was similar between most of the sites when represented in Euclidean space. The number of dominant denitrifiers detected for each sample ranged between 6 and 18 for nirK, 4-10 for nirS and 6-17 for nosZ. The seventy-two sequenced phylotypes showed 80-98 % identity to previously reported environmental sequences from water column, sediments and soil samples. These results suggest that Antarctic microbial mats have a large denitrification potential, previously uncharacterized and composed by both site-specific and common phylotypes belonging mainly to Alpha-, Beta- and Gammaproteobacteria.
机译：蓝细菌微生物垫是高度结构化的群落，通常在南极内陆水域（包括熔体流）中发现。这些底栖微生物协会包括大量具有不同代谢能力的微生物，从而影响了养分动态。反硝化过程是可行的氮损失途径，也是一氧化二氮的生物来源，一氧化二氮是一种强力的温室气体，也可促进臭氧消耗。使用PCR-DGGE（变性梯度凝胶电泳）方法对来自五个微生物垫的潜在反硝化剂进行了表征。使用了在反硝化过程中编码关键酶的分子标记（nirK，nirS和nosZ）。获得了五个采样垫的指纹，并连续两年进行了比较。距离分析显示，尽管采样年份不同，但以欧几里得空间表示时，大多数站点之间的反硝化遗传潜力相似。每个样品检测到的主要反硝化剂的数量在nirK的6到18之间，nirS的4-10在和nosZ 6-17之间。 72种测序的系统型与先前报道的来自水柱，沉积物和土壤样品的环境序列显示出80-98％的同一性。这些结果表明，南极微生物垫具有很大的反硝化潜能，以前未被表征，并且由主要属于α，β和γ蛋白菌的位点特异性和常见系统型组成。

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  • 来源
    《Polar biology》 |2014年第3期|403-416|共14页
  • 作者

    Rocio J. Alcantara-Hernandez; Carla M. Centeno; Alejandro Ponce-Mendoza; Silvia Batista; Martin Merino-Ibarra; Julio Campo; Luisa I. Falcon;

  • 作者单位

    Laboratorio de Ecologia Bacteriana y Epigenetica, Instituto de Ecologia, Universidad Nacional Autonoma de Mexico, 3er Circuito Exterior sn. Ciudad Universitaria, Del. Coyoacan, 04510 Mexico, DF, Mexico;

    Centra Nacional de Investigacion Disciplinaria en Conservation y Mejoramiento de Ecosistemas Forestales, INIFAP, Mexico, Mexico;

    Centra Nacional de Investigacion Disciplinaria en Conservation y Mejoramiento de Ecosistemas Forestales, INIFAP, Mexico, Mexico;

    Unidad de Microbiologia Molecular, Instituto de Investigaciones Biologicas Clemente Estable (MEC), Montevideo, Uruguay;

    Unidad Academica de Ecologia Marina, Instituto de Ciencias del Mar y Limnologia, Universidad Nacional Autonoma de Mexico, Mexico, DF, Mexico;

    Laboratorio de Biogeoquimica Terrestre y Clima, Instituto de Ecologia, Universidad Nacional Autonoma de Mexico, Mexico, DF, Mexico;

    Laboratorio de Ecologia Bacteriana y Epigenetica, Instituto de Ecologia, Universidad Nacional Autonoma de Mexico, 3er Circuito Exterior sn. Ciudad Universitaria, Del. Coyoacan, 04510 Mexico, DF, Mexico;

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  • 正文语种 eng
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  • 关键词

    Cyanobacterial microbial mat; Denitrifiers; PCR-DGGE; Maritime Antarctica;

    机译：蓝细菌微生物垫;反硝化剂PCR-DGGE;海上南极洲;

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