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首页> 外文期刊>Polar biology >Tubulin folding: the special case of a beta-tubulin isotype from the Antarctic psychrophilic ciliate Euplotes focardii
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Tubulin folding: the special case of a beta-tubulin isotype from the Antarctic psychrophilic ciliate Euplotes focardii

机译：小管蛋白折叠：来自南极嗜热纤毛虫Eupplotes focardii的β-微管蛋白同种型的特殊情况

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摘要

Folding assistance is a fundamental requirement of certain proteins, and it may be subjected to physicochemical constraints in case of organisms adapted to polar temperatures. Limited information is available about protein folding in the polar environment. Folding of tubulin provides one of the few studied cases. Here, we report a pilot folding analysis of a divergent beta-tubulin isotype, named EFBT3, from the Antarctic psychrophilic ciliate Euplotes focardii. To attain its native monomeric structure, beta-tubulin needs the assistance of the eukary-otic class II chaperonin CCT and cofactor A (CofA). The in vitro folding reaction of EFBT3 with CCT and CofA purified from rabbit did not generate any folded product. In contrast, the reaction performed with the rabbit reticulocyte lysate, that contains all the chaperones required for efficient tubulin folding, was productive, suggesting that additional factors besides purified CCT and CofA are required for EFBT3 to attain its monomeric structure. We also demonstrated that the rare Cys281 of EFBT3 is critical for the folding reaction. Model predictions indicate that EFBT3 binds to CofA differently from yeast beta-tubulin, suggesting a diverse folding mechanism that may be correlated with microtubule cold adaptation.
机译：折叠辅助是某些蛋白质的基本要求，在生物适应极温的情况下，折叠辅助可能会受到物理化学的约束。关于极地环境中蛋白质折叠的信息有限。微管蛋白折叠提供了少数研究案例之一。在这里，我们报告了来自南极嗜冷纤毛虫Euplotes focardii的不同β-微管蛋白同种型，名为EFBT3的试点折叠分析。为了获得其天然的单体结构，β-微管蛋白需要真核II类分子伴侣CCT和辅因子A（CofA）的协助。 EFBT3与兔纯化的CCT和CofA的体外折叠反应未产生任何折叠产物。相反，用兔网织红细胞裂解物进行的反应是有效的，其中含有有效微管蛋白折叠所需的所有分子伴侣，这表明EFBT3除了纯化的CCT和CofA以外，还需要其他因素才能获得其单体结构。我们还证明了EFBT3的罕见Cys281对折叠反应至关重要。模型预测表明，EFBT3与酵母β-微管蛋白的结合方式与CofA不同，这表明可能与微管冷适应相关的多种折叠机制。

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  • 来源
    《Polar biology》 |2013年第12期|1833-1838|共6页
  • 作者

    Sandra Pucciarelli; Federica Chiappori; Daniela Sparvoli; Luciano Milanesi; Cristina Miceli; Ronald Melki;

  • 作者单位

    School of Biosciences and Biotechnology, University of Camerino, Via Gentile III da Varano 1,62032 Camerino, MC. Italy;

    Istituto di Tecnologie Biomediche Consiglio Nazionale delle Ricerche, Segrate, MI, Italy;

    School of Biosciences and Biotechnology, University of Camerino, Via Gentile III da Varano 1,62032 Camerino, MC. Italy;

    Istituto di Tecnologie Biomediche Consiglio Nazionale delle Ricerche, Segrate, MI, Italy;

    School of Biosciences and Biotechnology, University of Camerino, Via Gentile III da Varano 1,62032 Camerino, MC. Italy;

    Laboratoire d'Enzymologie et Biochimie Structurales,Centre National de la Recherche Scientifique,91198 Gif-sur-Yvette, France;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Protozoan; Molecular chaperons; Site-directed mutagenesis; Modeling; Cold adaptation; Tubulin cofactor A;

    机译：原虫;分子伴侣;定点诱变;造型;冷适应;微管蛋白辅因子A;

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