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首页> 外文期刊>Polish Journal of Environmental Studies >Pollutant Removal and PCR-DGGE Analysis of Microbial Community Structural Changes in an Integrated Vertical-Flow Constructed Wetland
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Pollutant Removal and PCR-DGGE Analysis of Microbial Community Structural Changes in an Integrated Vertical-Flow Constructed Wetland

机译：垂直流人工湿地中微生物群落结构变化的污染物去除及PCR-DGGE分析

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In this study we measured changes in the CODCr, TN, NH4+-N, TP, and DO concentrations and the pH of different layers in an integrated vertical-flow constructed wetland (IVCW) treating eutrophic water. We also examined the composition and diversity of the bacterial community in the IVCW using PCR-DGGE. Our results showed that most of the contaminants were removed during sewage flow from the down-flow chamber to the up-flow chamber. The removal rates of TN, TP, CODCr, and NH4+-N were, respectively, 63.7%, 66.7%, 72.2%, and 67.9% in the down-flow; consequently, the CODCr and TP concentrations of the effluent fell below 10 mg/L and 0.05 mg/L. There were some common microorganisms and specific microorganisms in the different layers of the constructed wetland because of the changes in environmental factors and nutrient levels. The diversity of the bacterial community was highest in the upper layer of the down-flow system, and the similarity in the down-flow system was higher than that in the up-flow system. From the down-flow system to the up-flow system, the diversity of the bacterial community increased from 2.31 to 1.95 and the structural similarity gradually increased from 65.7% to 70.9%. The higher complexity of the bacterial community in the upper layer of the constructed wetland may be supported by plant oxygenation, rhizodeposition, and litter accumulation.
机译：在这项研究中，我们测量了处理富营养化水的集成垂直流人工湿地（IVCW）中CODCr，TN，NH4 + -N，TP和DO浓度以及不同层的pH的变化。我们还使用PCR-DGGE检查了IVCW中细菌群落的组成和多样性。我们的结果表明，在污水从下流室流向上流室的过程中，大部分污染物被去除。下降流中TN，TP，CODCr和NH4 + -N的去除率分别为63.7％，66.7％，72.2％和67.9％。因此，出水的CODCr和TP浓度分别低于10 mg / L和0.05 mg / L。由于环境因素和养分水平的变化，在人工湿地的不同层中存在一些常见的微生物和特定的微生物。细菌群落的多样性在下流系统的上层最高，而下流系统的相似性高于上流系统。从下流系统到上流系统，细菌群落的多样性从2.31增加到1.95，结构相似性从65.7％逐渐增加到70.9％。人工湿地上层中细菌群落的更高复杂性可以通过植物的氧合作用，根状沉积和凋落物的积累来支持。

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