• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the Arkansas Academy of Science >Effect of Thidiazuron and Benzylaminopurine on In Vitro Shoot Proliferation of Carnation (Dianthus caryophyllus L.)
【24h】

Effect of Thidiazuron and Benzylaminopurine on In Vitro Shoot Proliferation of Carnation (Dianthus caryophyllus L.)

机译：噻二唑酮和苄氨基嘌呤对康乃馨离体芽增殖的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Carnations (Dianthus caryophyllus L.) are among the most widely used cut flowers in the world. Tissue culture techniques offer an efficient method for the micropropagation of carnations. This study was conducted to test the effect of thidiazuron (TDZ) and benzylaminopurine (BAP), artificial cytokinins, on shoot multiplication of two carnation cultivars, Barlo II Nora and Raggio di Sole. Isolated axillary buds were cultured on Gamborg's (B-5) basal medium supplemented with 30 g/L sucrose and 8 g/L agar. The cultures were maintained at a 10-h photoperiod (40 μEm~(-2)s~(-1)) and 23℃ ± 2℃. Number of multiple shoots produced was dependent upon the genotype and was also influenced by the cytokinin type and concentration. Barlo II Nora produced the highest shoot number with 14 shoots per explant on a medium containing 20 mg/L BAP. The cultivar Raggio di Sole cultured on BAP-containing media produced a maximum of 4 shoots per explant. Barlo II Nora cultured on TDZ-containing media produced a maximum of 8 shoots per explant, however, large amounts of calli were associated with these shoots. Increasing the concentration of cytokinin was associated with an increase in shoot number and a decrease in shoot height. Shoots were rooted on Gamborg's medium containing 2 mg/L of 3-indole-butyric acid (IBA) and then transferred to pots. Once acclimatized the carnations were transferred to a greenhouse where they exhibited normal growth. This method could be useful for the rapid propagation of carnations in commercial production.
机译：康乃馨（Dianthus caryophyllus L.）是世界上使用最广泛的切花之一。组织培养技术为康乃馨的微繁殖提供了一种有效的方法。进行了这项研究，以测试噻菌灵（TDZ）和苄基氨基嘌呤（BAP）（人工细胞分裂素）对两个康乃馨品种Barlo II Nora和Raggio di Sole的芽增殖的影响。将分离的腋芽在补充有30 g / L蔗糖和8 g / L琼脂的Gamborg（B-5）基础培养基上培养。将培养物维持在10小时的光周期（40μEm〜（-2）s〜（-1））和23℃±2℃。产生的多次芽的数量取决于基因型，并且还受细胞分裂素类型和浓度的影响。在含有20 mg / L BAP的培养基上，Barlo II Nora产生最高的芽数，每个外植体有14芽。在含BAP的培养基上培养的Raggio di Sole品种每个外植体最多产生4个芽。在含TDZ的培养基上培养的Barlo II Nora每株外植体最多可产生8个芽，但是，大量的愈伤组织与这些芽相关。细胞分裂素浓度的增加与芽数的增加和芽高的降低有关。将芽生根于含有2 mg / L 3-吲哚丁酸（IBA）的Gamborg培养基中，然后转移到盆中。一旦适应，康乃馨被转移到温室中，在那里它们表现出正常的生长。该方法对于康乃馨在商业生产中的快速繁殖可能是有用的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号