Rapid induction of tumor necrosis factor alpha in the cerebrospinal fluid after intracerebroventricular injection of lipopolysaccharide revealed by a sensitive capture immuno-PCR assay.

Sanna PP; Weiss F; Samson ME; Bloom FE; Pich EM

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Rapid induction of tumor necrosis factor alpha in the cerebrospinal fluid after intracerebroventricular injection of lipopolysaccharide revealed by a sensitive capture immuno-PCR assay.

机译：脑室注射脂多糖后脑脊液中肿瘤坏死因子α的快速诱导通过灵敏的捕获免疫PCR分析显示。

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摘要

Tumor necrosis factor alpha (TNF-alpha) is an important mediator in many pathophysiologic processes, both in the central nervous system (CNS) and in the periphery. For this study, we have designed a very sensitive immuno-PCR detection system to investigate the time course of TNF-alpha induction in the rat cerebrospinal fluid after intracerebroventricular administration of bacterial lipopolysaccharide (LPS). Immuno-PCR combines antibody specificity with PCR signal amplification and provides a sensitivity in the picomolar range. The enhanced sensitivity of this assay allowed the detection of TNF-alpha in the cerebrospinal fluid as early as 15 min after intracerebroventricular administration of LPS. The present results suggest that the ventricular compartment of the CNS, although confined within the blood-brain barrier, is highly responsive to proinflammatory stimuli such as LPS administration. Insight into the molecular mechanisms underlying this compartmentalization could be key to the pathology and treatment of many CNS diseases, especially the meningitides.

机译：肿瘤坏死因子α（TNF-alpha）是中枢神经系统（CNS）和周围许多病理生理过程中的重要介体。对于本研究，我们设计了一个非常灵敏的免疫PCR检测系统，以研究脑室内给予细菌性脂多糖（LPS）后大鼠脑脊液中TNF-α诱导的时间过程。 Immuno-PCR将抗体特异性与PCR信号扩增相结合，并提供了皮摩尔级的灵敏度。该测定法提高了灵敏度，可在脑室内给予LPS后15分钟之内检测到脑脊液中的TNF-α。目前的结果表明，尽管CNS的室间隔被限制在血脑屏障内，但是它对诸如LPS施用的促炎性刺激高度响应。深入了解这种分隔的分子机制可能是许多中枢神经系统疾病（尤其是脑膜炎）的病理和治疗的关键。

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1995年第1期|P.272-275|共4页
作者
Sanna PP; Weiss F; Samson ME; Bloom FE; Pich EM;
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作者单位

Department of Neuropharmacology, Scripps Research Institute, La Jolla, CA 92037.;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Cerebral Ventricles; Lipopolysaccharides; Polymerase Chain Reaction; Tumor Necrosis Factor; 脑室; 脂多糖类; 聚合酶链反应; 肿瘤坏死因子;

机译：Cerebral Ventricles;Lipopolysaccharides;Polymerase Chain Reaction;Tumor Necrosis Factor;脑室;脂多糖类;聚合酶链反应;肿瘤坏死因子;

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1. Blood interleukin-6 and tumor necrosis factor-alpha elevation after intracerebroventricular injection of Escherichia coli endotoxin in the rat is determined by two opposing factors: peripheral induction by LPS transferred from brain to blood and inhi [J] . Chen G, McCuskey RS, Reichlin S Neuroimmunomodulation . 2000,第2期

机译：大鼠脑室内注射大肠杆菌内毒素后血液中白细胞介素6和肿瘤坏死因子-α的升高是由两个相反的因素决定的：脂多糖从脑转移到血液中的外周诱导和抑制
2. Nonparticipation of nuclear factor kappa B (NFkappaB) in the signaling cascade of c-Jun N-terminal kinase (JNK)- and p38 mitogen-activated protein kinase (p38MAPK)-dependent tumor necrosis factor alpha (TNFalpha) induction in lipopolysaccharide (LPS) [J] . Uesugi M, Nakajima K, Tohyama Y, Brain research . 2006,第0期

机译：脂多糖（LPS）中c-Jun N端激酶（JNK）和p38丝裂原活化蛋白激酶（p38MAPK）依赖性肿瘤坏死因子α（TNFalpha）的信号级联反应中不参与核因子kappa B（NFkappaB）的诱导
3. alpha-melanocyte-stimulating hormone modulates lipopolysaccharide plus interferon-gamma-induced tumor necrosis factor-alpha expression but not tumor necrosis factor-alpha receptor expression in cultured hypothalamic neurons. [J] . Caruso C, Sanchez M, Durand D, Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology . 2010,第1a2期

机译：在培养的下丘脑神经元中，α-刺激黑素细胞的激素调节脂多糖加干扰素-γ诱导的肿瘤坏死因子-α表达，但不调节肿瘤坏死因子-α受体表达。
4. Inhibitory Effects of a Rice Hull Constituent on Tumor Necrosis Factor, Prostaglandin E2, and Cyclooxygenase-2 Production in Lipopolysaccharide-Activated Mouse Macrophages [C] . SHENG-TUNG HUANG, CHIEN-TSU CHEN, KUR-TA CHIENG, Asian Society for Mitochondrial Research and Medicine . 2005

机译：稻壳组分对脂多糖活化小鼠巨噬细胞肿瘤坏死因子，前列腺素E2和环氧氧酶-2生产的抑制作用
5. A distinct element involved in the lipopolysaccharide activation of the tumor necrosis factor-alpha promoter in a promonocytic cell line, THP-1. [D] . Diaz, Belisa. 1999

机译：在原单核细胞系THP-1中，与肿瘤坏死因子-α启动子的脂多糖活化有关的独特元素。
6. Rapid induction of tumor necrosis factor alpha in the cerebrospinal fluid after intracerebroventricular injection of lipopolysaccharide revealed by a sensitive capture immuno-PCR assay. [O] . P P Sanna, F Weiss, M E Samson, 1995

机译：脑室注射脂多糖后脑脊液中肿瘤坏死因子α的快速诱导通过灵敏的捕获免疫PCR分析显示。
7. Rapid induction of tumor necrosis factor alpha in the cerebrospinal fluid after intracerebroventricular injection of lipopolysaccharide revealed by a sensitive capture immuno-PCR assay. [O] . Sanna, P P, Weiss, F, Samson, M E, 1995

机译：脑室注射脂多糖后脑脊液中肿瘤坏死因子α的快速诱导通过灵敏的捕获免疫PCR分析显示。
8. Induction of Colony Stimulating Factor In Vivo by Recombinant Interleukin 1 Alpha and Recombinant Tumor Necrosis Factor Alpha [R] . Vogel, S. N., Douches, S. D., Kaufman, E. N., 1987

机译：重组白细胞介素1α和重组肿瘤坏死因子α在体内诱导集落刺激因子

Rapid induction of tumor necrosis factor alpha in the cerebrospinal fluid after intracerebroventricular injection of lipopolysaccharide revealed by a sensitive capture immuno-PCR assay.

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