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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Cell cycle regulation of metallothionein in human colonic cancer cells.
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Cell cycle regulation of metallothionein in human colonic cancer cells.

机译：人结肠癌细胞中金属硫蛋白的细胞周期调节。

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摘要

Elevated levels of metallothionein (MT) found in rapidly growing tissues such as neonatal liver and various types of human tumors have suggested a role for MT in cell proliferation. To further explore this possibility we investigated the concentration of MT in human colonic cancer (HT-29) cells at different stages of proliferation by means of immunocytochemistry and competitive binding. MT is increased in subconfluent proliferating cells relative to growth-inhibited confluent cells, much as it is in growing tissues. Cycling cells synchronized with compactin, an inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, revealed an oscillation of cytoplasmic MT that reached a maximum in successive late G1 phases and at the G1/S transition. Individual phase of the cell cycle were assessed by [3H]thymidine incorporation and by immunofluorescence employing an antibody that detects a nuclear antigen associated with proliferation. An enzyme-linked immunosorbent assay was used to quantify the relative amounts of MT in homogenate supernatants of HT-29 cells. A 2- to 3-fold increase in MT in actively proliferating cells and the regulation of the protein during the mitotic cell cycle point to a physiological role for MT in cellular proliferation and suggest that it may also serve as a proliferation marker.
机译：在快速生长的组织（如新生儿肝脏）和各种类型的人类肿瘤中发现的金属硫蛋白（MT）水平升高，表明MT在细胞增殖中发挥了作用。为了进一步探讨这种可能性，我们通过免疫细胞化学和竞争结合的方法研究了人结肠癌（HT-29）细胞在不同增殖阶段的MT浓度。相对于生长抑制的汇合细胞，亚汇合增殖细胞中的MT升高，这与生长组织中的MT一样。循环细胞与3-羟基-3-甲基戊二酰辅酶A还原酶抑制剂Compactin同步，揭示了胞质MT的振荡，该振荡在连续的后期G1期和G1 / S过渡期达到最大值。通过[3 H]胸苷掺入和通过使用检测与增殖相关的核抗原的抗体的免疫荧光来评估细胞周期的各个阶段。使用酶联免疫吸附测定法定量HT-29细胞匀浆上清液中MT的相对量。活跃增殖细胞中MT的2到3倍增加以及有丝分裂细胞周期中蛋白质的调节表明MT在细胞增殖中的生理作用，并暗示它也可以用作增殖标记。

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