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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >CD18 (β_2 leukocyte integrin) promoter requires PU.1 transcription factor for myeloid activity
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CD18 (β_2 leukocyte integrin) promoter requires PU.1 transcription factor for myeloid activity

机译：CD18（β_2白细胞整合素）启动子需要PU.1转录因子才能实现髓样活性

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摘要

Normal cellular differentiation is linked to tightly regulated gene transcription. However, the DNA elements and trans-acting factors that regulate transcription in myeloid cells are poorly defined. CD18, the β chain of the leukocyte integrins, is transcriptionally regulated during myeloid differentiation. The CD18 promoter is active after trans-fection into myeloid cells. We demonstrate that a region of the CD18 promoter that contains two binding sites for the PU.1 transcription factor is required for activity in myeloid cells. These sites are bound by in vitro translated PU.1 and by PU.1 from myeloid nuclear extracts. Mutagenesis of these sites abrogates binding by PU.1 and substantially decreases promoter activity in myeloid cells. Thus, the leukocyte-specific transcription factor PU.1 is required for myeloid activity of CD18.
机译：正常的细胞分化与严格调控的基因转录有关。但是，调节髓样细胞转录的DNA元素和反式作用因子定义不清。 CD18是白细胞整合素的β链，在髓样分化过程中受到转录调控。转染入髓样细胞后，CD18启动子具有活性。我们证明CD18启动子的区域包含两个PU.1转录因子的结合位点是髓细胞中活性所必需的。这些位点被体外翻译的PU.1和髓核提取物中的PU.1结合。这些位点的诱变消除了PU.1的结合，并大大降低了髓样细胞中的启动子活性。因此，CD18的髓样活性需要白细胞特异性转录因子PU.1。

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