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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Induction of heme oxygenase 1 in the retina by intense visible light: suppression by the antioxidant dimethylthiourea.
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Induction of heme oxygenase 1 in the retina by intense visible light: suppression by the antioxidant dimethylthiourea.

机译：强烈的可见光在视网膜中诱导血红素加氧酶1：被抗氧化剂二甲基硫脲抑制。

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摘要

The effect of intense visible light (light damage) on the expression of heme oxygenase 1 (HO-1), a protein induced by oxidative stress, was investigated in the rat retina. A sensitive reverse transcription-PCR assay demonstrated the expression of mRNA for HO-1 as well as HO-2, the noninducible HO form, in the normal retina. As analyzed by Northern blotting, however, HO-1 mRNA was barely detectable under normal circumstances. After exposure to intense visible light, retinas had markedly higher HO-1 mRNA levels than unexposed controls, with increases up to 52- and 98-fold at 12 and 24 hr of exposure, respectively. Intense light exposure also resulted in an increase in HO-1 protein. In contrast, no appreciable change in HO-2 mRNA or protein was observed. The increase in HO-1 message was more pronounced in rats previously reared in the dark than in those reared in a weak cyclic-light environment. A marked decrease from the high level of HO-1 mRNA induced by light insult was observed when the animals were allowed to recover in the dark for 24 hr after light exposure. Most important, treatment of animals with 1,3-dimethylthiourea, a synthetic antioxidant, prior to light exposure effectively blocked the increase in HO-1 mRNA. Thus, HO-1 is a sensitive marker for assessing light-induced insult in the retina. Since increased expression of HO-1 is thought to be a cellular defense against oxidative damage, its expression may play an important role in protecting the retina against light damage.
机译：在大鼠视网膜中研究了强烈的可见光（光损伤）对血红素加氧酶1（HO-1）（一种由氧化应激诱导的蛋白质）表达的影响。敏感的逆转录-PCR分析表明，正常视网膜中HO-1和HO-2（非诱导型HO形式）的mRNA表达。然而，通过Northern印迹分析，在正常情况下几乎检测不到HO-1 mRNA。暴露于强烈的可见光后，视网膜的HO-1 mRNA水平显着高于未暴露的对照，在暴露12和24小时时，其HO-1 mRNA水平分别增加了52倍和98倍。强烈的光照也导致HO-1蛋白增加。相反，未观察到HO-2 mRNA或蛋白质的明显变化。以前在黑暗中饲养的大鼠中HO-1信息的增加比在弱循环光环境中饲养的大鼠中更明显。当使动物在光照后24小时在黑暗中恢复时，观察到由光损伤诱导的HO-1 mRNA的高水平明显降低。最重要的是，在曝光前用1,3-二甲基硫脲（一种合成抗氧化剂）对动物进行治疗有效地阻止了HO-1 mRNA的升高。因此，HO-1是用于评估光诱导的视网膜损伤的敏感标记。由于HO-1的表达增加被认为是抗氧化损伤的细胞防御，因此其表达可能在保护视网膜免受光损伤中起重要作用。

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