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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Efficient induction of point mutations allowing recovery of specific locus mutations in zebrafish.
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Efficient induction of point mutations allowing recovery of specific locus mutations in zebrafish.

机译：点突变的有效诱导，可以恢复斑马鱼中的特定基因座突变。

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摘要

A technique is described that greatly increases the efficiency of recovering specific locus point mutations in zebrafish (Danio rerio). Founder individuals that were mosaic for point mutations were produced by mutagenizing postmeiotic gametes with the alkylating agent N-ethyl-N-nitrosourea. Under optimal conditions, each founder carried an average of 10 mutations affecting genes required for embryogenesis. Moreover, approximately 2% of these founders transmitted new mutations at any prespecified pigmentation locus. Analyses of new pigmentation mutations confirmed that most were likely to be point mutations. Thus, mutagenesis of postmeiotic gametes with N-ethyl-N-nitrosourea yielded frequencies of point mutations at specific loci that were 10- to 15-fold higher than previously achieved in zebrafish. Our procedure should, therefore, greatly facilitate recovery of multiple mutant alleles at any locus of interest.
机译：描述了一种技术，该技术可大大提高恢复斑马鱼（Danio rerio）中特定基因座点突变的效率。通过用烷基化剂N-乙基-N-亚硝基脲诱变减数分裂后的配子产生了点突变镶嵌的奠基人。在最佳条件下，每个创建者平均携带10个影响胚胎发生所需基因的突变。此外，这些创建者中约有2％在任何预先指定的色素沉着位点传播了新的突变。对新的色素沉着突变的分析证实，大多数可能是点突变。因此，用N-乙基-N-亚硝基脲诱变减数分裂配子后，在特定基因座的点突变频率比斑马鱼以前的基因突变高10至15倍。因此，我们的程序应极大地促进在任何目的基因座处恢复多个突变等位基因。

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