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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Molecular cloning and expression of the 32-kDa subunit of human TFIID reveals interactions with VP16 and TFIIB that mediate transcriptional activation.
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Molecular cloning and expression of the 32-kDa subunit of human TFIID reveals interactions with VP16 and TFIIB that mediate transcriptional activation.

机译：人TFIID的32 kDa亚基的分子克隆和表达揭示了与介导转录激活的VP16和TFIIB的相互作用。

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摘要

Transcription factor TFIID consists of TATA binding protein (TBP) and at least eight TBP-associated factors (TAFs). As TAFs are required for activated but not basal transcription, we have proposed that TAFs act as coactivators to mediate signals between activators and the basal transcription machinery. Here we report the cloning, expression, and biochemical characterization of the 32-kDa subunit of human (h) TFIID, termed hTAFII32. We find that hTAFII32 is the human homologue of Drosophila TAFII40. In vitro protein-protein interaction assays reveal that as observed with Drosophila TAFII40, hTAFII32 interacts with the C-terminal 39-amino acid activation domain of the acidic transactivator viral protein 16 (VP16) as well as with the general transcription factor TFIIB. Moreover, a partial recombinant TFIID complex containing hTAFII32 was capable of mediating in vitro transcriptional activation by the VP16 activation domain. These findings indicate that specific activator-coactivator interactions have beenconserved between human and Drosophila and provide additional support for the function of these interactions in mediating transcriptional activation.
机译：转录因子TFIID由TATA结合蛋白（TBP）和至少八个TBP相关因子（TAF）组成。由于激活需要TAF，但基础转录不是必需的，因此我们建议TAF作为共激活因子来介导激活因子和基础转录机制之间的信号。在这里，我们报告人类（h）TFIID，称为hTAFII32的32 kDa亚基的克隆，表达和生化特征。我们发现hTAFII32是果蝇TAFII40的人类同源物。体外蛋白质-蛋白质相互作用测定揭示，如用果蝇TAFII40观察到的，hTAFII32与酸性反式激活蛋白16（VP16）的C末端39个氨基酸激活结构域以及一般转录因子TFIBB相互作用。而且，含有hTAFII32的部分重组TFIID复合物能够通过VP16激活结构域介导体外转录激活。这些发现表明，人与果蝇之间已经保留了特定的激活剂-共激活剂相互作用，并为这些相互作用在介导转录激活中的功能提供了额外的支持。

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